Yeast Protocols

  • Wei Xiao

Part of the Methods in Molecular Biology book series (MIMB, volume 1163)

Table of contents

  1. Front Matter
    Pages i-x
  2. Brendan P. G. Curran, Virginia Bugeja
    Pages 1-14
  3. Michael Biss, Michelle D. Hanna, Wei Xiao
    Pages 15-21
  4. Johannes H. Hegemann, Sven Boris Heick, Jennifer Pöhlmann, Marcus M. Langen, Ursula Fleig
    Pages 45-73
  5. Elisa Donnard, Erica M. Queiroz, J. Miguel Ortega, R. Daniel Gietz
    Pages 97-107
  6. Likui Zhang, Alina Radziwon, Linda J. Reha-Krantz
    Pages 109-129
  7. Wei-Pang Huang, Takahiro Shintani, Zhiping Xie
    Pages 153-164
  8. Tomotake Kanki, Koji Okamoto
    Pages 165-173
  9. Xin Xu, Amanda D. Lambrecht, Wei Xiao
    Pages 183-191
  10. Susan Blackwell, Michelle D. Hanna, Wei Xiao
    Pages 193-199
  11. Spike D. L. Postnikoff, Troy A. A. Harkness
    Pages 223-227
  12. Christopher A. Jackson, Michael C. Yu
    Pages 229-247

About this book

Introduction

Yeast Protocols, Third Edition presents up-to-date advances in research using yeasts as models. Chapters cover topics such as basic protocols in yeast culture and genomic manipulation, protocols that study certain organelles such as mitochondria and peroxisomes and their functions in autophagy, and assays commonly used in yeast-based studies that can be adapted to other organisms. As the first sequenced living organism, budding yeast S. cerevisiae and other model yeasts have helped greatly in life science research. The easy switch between the haploid and diploid state makes yeast a paradigm of genetic manipulation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Yeast Protocols, Third Edition seeks to serve both professionals and novices with newly-developed protocols to study this essential model organism.

Keywords

eukaryotic organisms flow cytometry gene manipulation growth assays yeast models

Editors and affiliations

  • Wei Xiao
    • 1
  1. 1.Capital Normal University College of Life SciencesBeijingChina

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-0799-1
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-0798-4
  • Online ISBN 978-1-4939-0799-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book