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Investigating RNAs Involved in Translational Control by NMR and SAXS

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Biophysical approaches to translational control of gene expression

Part of the book series: Biophysics for the Life Sciences ((BIOPHYS,volume 1))

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Abstract

Nuclear magnetic resonance (NMR) spectroscopy and small angle X-ray scattering (SAXS) are solution-based methods that can be used to analyze and determine molecular structure. Here, we review the use of NMR and SAXS for the structural analysis of RNA molecules, with a focus on RNAs involved in translational control. Practical considerations, experimental requirements, and data analysis are discussed. We also review a recently developed hybrid approach that combines NMR and SAXS. The hybrid NMR-SAXS approach shows great promise for expanding the scope of biophysical studies on biological molecules in solution. Finally, we review examples of these methods applied to RNA systems such as programmed ribosomal frameshift sites, internal ribosome entry sites, ribosome-binding structural elements, RNA thermometers, and riboswitches.

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  1. Department of Biochemistry, University of Wisconsin, Madison, WI, USA

    Kathryn D. Mouzakis, Jordan E. Burke & Samuel E. Butcher

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  1. Kathryn D. Mouzakis
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  2. Jordan E. Burke
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  3. Samuel E. Butcher
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Correspondence to Samuel E. Butcher .

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  1. College Park, Cell Biology and Molecular Genetics, University of Maryland, Rm. 2135 Microbiology Building, College Park, 20742-4451, Maryland, USA

    Jonathan D. Dinman

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Mouzakis, K.D., Burke, J.E., Butcher, S.E. (2012). Investigating RNAs Involved in Translational Control by NMR and SAXS. In: Dinman, J. (eds) Biophysical approaches to translational control of gene expression. Biophysics for the Life Sciences, vol 1. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-3991-2_8

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