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Lineage Tracing of Bone Cells in the Regenerating Fin and During Repair of Bone Lesions

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Zebrafish

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2707))

Abstract

Small teleost fishes such as zebrafish and medaka show remarkable regeneration capabilities upon tissue injury or amputation. To elucidate cellular mechanisms of teleost tissue repair and regeneration processes, the Cre/LoxP recombination system for cell lineage tracing is a widely used technique. In this chapter, we describe protocols used for inducible Cre/LoxP recombination-mediated lineage tracing of osteoblast progenitors during medaka fin regeneration as well as during the repair of osteoporosis-like bone lesions in the medaka vertebral column. Our approach can be adapted for lineage tracing of other cell populations in the regenerating teleost fin or in other tissues undergoing repair.

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Acknowledgments

We thank Lazaro Centanin (Centre for Organismal Studies, University of Heidelberg) for sharing the GaudiBBW2.1 transgenic line. We also thank the Centre for Bioimaging Sciences confocal unit and the fish facility at Department of Biological Sciences, National University of Singapore, for continued support. This work was supported by the Singapore Ministry of Education (MOE2016-T2-2-086) and the National Research Foundation Singapore (NRF2017-NRF-ISF002-2671). The authors declare no competing interests.

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Authors and Affiliations

  1. Department of Biological Sciences and Centre for Bioimaging Sciences, National University of Singapore, Singapore, Singapore

    Christoph Winkler

  2. Department of Developmental Genetics, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany

    Wen Hui Tan

Authors
  1. Wen Hui Tan
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  2. Christoph Winkler
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Corresponding author

Correspondence to Wen Hui Tan .

Editor information

Editors and Affiliations

  1. Department of Pediatrics Children’s Hospital Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA

    James F. Amatruda

  2. Centre for Developmental Neurobiology, MRC Centre for NeuroDevelopmental Disorders, King’s College London, London, UK

    Corinne Houart

  3. Laboratory of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Shizuoka, Japan

    Koichi Kawakami

  4. Duke Regeneration Center, Department of Cell Biology, Duke University Medical Center, Durham, NC, USA

    Kenneth D. Poss

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Tan, W.H., Winkler, C. (2024). Lineage Tracing of Bone Cells in the Regenerating Fin and During Repair of Bone Lesions. In: Amatruda, J.F., Houart, C., Kawakami, K., Poss, K.D. (eds) Zebrafish. Methods in Molecular Biology, vol 2707. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3401-1_6

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  • DOI: https://doi.org/10.1007/978-1-0716-3401-1_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3400-4

  • Online ISBN: 978-1-0716-3401-1

  • eBook Packages: Springer Protocols

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