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Zebrafish

Methods and Protocols

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  • © 2024
  • Latest edition

Overview

Editors:
  1. James F. Amatruda

    1. Department of Pediatrics Children’s Hospital Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, USA

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  2. Corinne Houart

    1. Centre for Developmental Neurobiology, MRC Centre for NeuroDevelopmental Disorders, King’s College London, London, UK

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  3. Koichi Kawakami

    1. Laboratory of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Japan

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  4. Kenneth D. Poss

    1. Duke Regeneration Center, Department of Cell Biology, Duke University Medical Center, Durham, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2707)

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About this book

This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the vertebrate nervous system, tools and approaches using zebrafish to study stem cell and regenerative biology, as well as techniques in genetics and genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Zebrafish: Methods and Protocols, Third Edition serves as an invaluable guide to propel advances in developmental biology, disease modeling, and regeneration research using zebrafish and medaka as model systems.

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiv
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  2. Disease Models

    1. Front Matter

      Pages 1-1
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    2. Delivering Traumatic Brain Injury to Larval Zebrafish

      • Taylor Gill, Laszlo F. Locskai, Alexander H. Burton, Hadeel Alyenbaawi, Travis Wheeler, Edward A. Burton et al.
      Pages 3-22

    3. Functional Genomics of Novel Rhabdomyosarcoma Fusion-Oncogenes Using Zebrafish

      • Matthew R. Kent, Katherine Silvius, Jack Kucinski, Delia Calderon, Genevieve C. Kendall
      Pages 23-41

    4. Methods to Study Liver Disease Using Zebrafish Larvae

      • Elena Magnani, Anjana Ramdas Nair, Ian McBain, Patrice Delaney, Jaime Chu, Kirsten C. Sadler
      Pages 43-69

    5. Developmental Toxicity Assessment Using Zebrafish-Based High-Throughput Screening

      • Subham Dasgupta, Michael T. Simonich, Robyn L. Tanguay
      Pages 71-82

    6. Cancer Modeling by Transgene Electroporation in Adult Zebrafish (TEAZ)

      • Emily Montal, Shruthy Suresh, Yilun Ma, Mohita M. Tagore, Richard M. White
      Pages 83-97

    7. Lineage Tracing of Bone Cells in the Regenerating Fin and During Repair of Bone Lesions

      • Wen Hui Tan, Christoph Winkler
      Pages 99-110

  3. Neuroscience

    1. Front Matter

      Pages 111-111
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    2. Optimized Primary Culture of Neuronal Populations for Subcellular Omics Applications

      • Richard Taylor, Corinne Houart
      Pages 113-124

    3. Holographic Optogenetic Activation of Neurons Eliciting Locomotion in Head-Embedded Larval Zebrafish

      • Xinyu Jia, Claire Wyart
      Pages 125-140

    4. Brain Imaging and Registration in Larval Zebrafish

      • Ashwin A. Bhandiwad, Tripti Gupta, Abhignya Subedi, Victoria Heigh, George A. Holmes, Harold A. Burgess
      Pages 141-153

    5. Simultaneous Behavioral and Neuronal Imaging by Tracking Microscopy

      • Drew N. Robson, Jennifer M. Li
      Pages 155-167

    6. Genetic Identification of Neural Circuits Essential for Active Avoidance Fear Conditioning in Adult Zebrafish

      • Pradeep Lal, Hideyuki Tanabe, Koichi Kawakami
      Pages 169-181
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Editors and Affiliations

  • Department of Pediatrics Children’s Hospital Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, USA

    James F. Amatruda

  • Centre for Developmental Neurobiology, MRC Centre for NeuroDevelopmental Disorders, King’s College London, London, UK

    Corinne Houart

  • Laboratory of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Japan

    Koichi Kawakami

  • Duke Regeneration Center, Department of Cell Biology, Duke University Medical Center, Durham, USA

    Kenneth D. Poss

Bibliographic Information

  • Book Title: Zebrafish

  • Book Subtitle: Methods and Protocols

  • Editors: James F. Amatruda, Corinne Houart, Koichi Kawakami, Kenneth D. Poss

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3401-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3400-4Published: 06 September 2023

  • Softcover ISBN: 978-1-0716-3403-5Due: 07 October 2023

  • eBook ISBN: 978-1-0716-3401-1Published: 05 September 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XIV, 360

  • Number of Illustrations: 9 b/w illustrations, 85 illustrations in colour

  • Topics: Biological Techniques, Biological Techniques

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