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Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors

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Flower Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2686))

Abstract

The flower is a hallmark feature that has contributed to the evolutionary success of land plants. Diverse mutagenic agents have been employed as a tool to genetically perturb flower development and identify genes involved in floral patterning and morphogenesis. Since the initial studies to identify genes governing processes such as floral organ specification, mutagenesis in sensitized backgrounds has been used to isolate enhancers and suppressors to further probe the molecular basis of floral development. Here, we first describe two commonly employed methods for mutagenesis (using ethyl methanesulfonate (EMS) or T-DNAs as mutagens), and then describe three methods for identifying a mutation that leads to phenotypic alterations: traditional map-based cloning, modified high-efficiency thermal asymmetric interlaced PCR (mhiTAIL-PCR), and deep sequencing in the plant model Arabidopsis thaliana.

This chapter is an updated version of Chapter 6 of Flower Development: Methods and Protocols (1st edition, 2014), Methods in Molecular Biology, vol 1110.

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Author information

Authors and Affiliations

  1. Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha, China

    Zhigang Huang

  2. Department of Botany and Plant Sciences, University of California, Riverside, CA, USA

    Thanh Theresa Dinh, Elizabeth Luscher, Shaofang Li, Xigang Liu, So Youn Won & Xuemei Chen

Authors
  1. Zhigang Huang
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  2. Thanh Theresa Dinh
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  3. Elizabeth Luscher
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  4. Shaofang Li
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  5. Xigang Liu
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  6. So Youn Won
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  7. Xuemei Chen
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Corresponding author

Correspondence to Xuemei Chen .

Editor information

Editors and Affiliations

  1. Centre for Research in Agricultural Genomics CSIC-IRTA-UAB-UB, Barcelona, Spain

    José Luis Riechmann

  2. Instituto de Biología Molecular y Celular de Plantas CSIC-UPV, Valencia, Spain

    Cristina Ferrándiz

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Huang, Z. et al. (2023). Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors. In: Riechmann, J.L., Ferrándiz, C. (eds) Flower Development . Methods in Molecular Biology, vol 2686. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3299-4_6

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  • DOI: https://doi.org/10.1007/978-1-0716-3299-4_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3298-7

  • Online ISBN: 978-1-0716-3299-4

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