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Design, Production, and Characterization of Catalytically Active Inclusion Bodies

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Inclusion Bodies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2617))

Abstract

Catalytically active inclusion bodies (CatIBs) are promising biologically produced enzyme/protein immobilizates for application in biocatalysis, synthetic chemistry, and biomedicine. CatIB formation is commonly induced by fusion of suitable aggregation-inducing tags to a given target protein. Heterologous production of the fusion protein in turn yields CatIBs. This chapter presents the methodology needed to design, produce, and characterize CatIBs.

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Acknowledgments

The research was financially supported by the CLIB Competence Center Biotechnology (CKB) funded by the European Regional Development Fund ERDF (34.EFRE 0300096 and 34.EFRE 0300097).

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Authors and Affiliations

  1. Institute of Molecular Enzyme Technology, Heinrich Heine University Düsseldorf, Forschungszentrum Jülich, Wilhelm-Johnen-Straße, Jülich, Germany

    Gizem Ölçücü, Karl-Erich Jaeger & Ulrich Krauss

  2. Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Wilhelm-Johnen-Straße, Jülich, Germany

    Gizem Ölçücü, Karl-Erich Jaeger & Ulrich Krauss

Authors
  1. Gizem Ölçücü
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  2. Karl-Erich Jaeger
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  3. Ulrich Krauss
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Corresponding authors

Correspondence to Gizem Ölçücü or Ulrich Krauss .

Editor information

Editors and Affiliations

  1. Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria

    Julian Kopp

  2. Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria

    Oliver Spadiut

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Ölçücü, G., Jaeger, KE., Krauss, U. (2023). Design, Production, and Characterization of Catalytically Active Inclusion Bodies. In: Kopp, J., Spadiut, O. (eds) Inclusion Bodies. Methods in Molecular Biology, vol 2617. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2930-7_4

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  • DOI: https://doi.org/10.1007/978-1-0716-2930-7_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2929-1

  • Online ISBN: 978-1-0716-2930-7

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