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Micro-CT–Based Bone Microarchitecture Analysis of the Murine Skull

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Craniofacial Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2403))

Abstract

X-ray micro-computed tomography (micro-CT) imaging has important applications in microarchitecture analysis of cortical and trabecular bone structure. While standardized protocols exist for micro-CT–based microarchitecture assessment of long bones, specific protocols need to be developed for different types of skull bones taking into account differences in embryogenesis, organization, development, and growth compared to the rest of the body. This chapter describes the general principles of bone microarchitecture analysis of murine craniofacial skeleton to accommodate for morphological variations in different regions of interest.

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Acknowledgments

This work has been supported by grants from the Australian Dental Research Foundation and the Australian Craniomaxillofacial Foundation.

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Authors and Affiliations

  1. Adelaide Dental School, The University of Adelaide, Adelaide, SA, Australia

    Jenny Tan, Mustafa Mian, Peter J. Anderson & Sarbin Ranjitkar

  2. Adelaide Microscopy, The University of Adelaide, Adelaide, SA, Australia

    Agatha Labrinidis & Ruth Williams

  3. Australian Craniofacial Unit, Women’s and Children’s Hospital, North Adelaide, SA, Australia

    Peter J. Anderson

  4. South Australian Health and Medical Research Institute (SAHMRI), Adelaide, SA, Australia

    Peter J. Anderson

  5. Department of Dentistry and Oral Health, La Trobe Rural Health School, La Trobe University, Bendigo, VIC, Australia

    Sarbin Ranjitkar

Authors
  1. Jenny Tan
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  2. Agatha Labrinidis
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  3. Ruth Williams
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  4. Mustafa Mian
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  5. Peter J. Anderson
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  6. Sarbin Ranjitkar
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Corresponding author

Correspondence to Sarbin Ranjitkar .

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Editors and Affiliations

  1. La Trobe University, Bundoora, VIC, Australia

    Sebastian Dworkin

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Tan, J., Labrinidis, A., Williams, R., Mian, M., Anderson, P.J., Ranjitkar, S. (2022). Micro-CT–Based Bone Microarchitecture Analysis of the Murine Skull. In: Dworkin, S. (eds) Craniofacial Development. Methods in Molecular Biology, vol 2403. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1847-9_10

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  • DOI: https://doi.org/10.1007/978-1-0716-1847-9_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1846-2

  • Online ISBN: 978-1-0716-1847-9

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