Abstract
Bacteria of the genus Streptomyces are one of the most important producers of biologically active natural products. Recent robust genomic sequencing of Streptomyces strains has shown enormous genetic potential for new natural products. However, many biosynthetic gene clusters are silent. Therefore, efficient and stable genome modification methods are needed to induce their production or to manipulate them for the production of new compounds or biotechnologically improved strains. We have recently developed a simple and efficient markerless genome modification system for these bacteria based on the positive selection of double crossovers using the blue pigment indigoidine bpsA gene. This chapter is an attempt to provide methodological details of this strategy for stable markerless genomic engineering (deletions/insertions) to improve their biotechnological properties and to produce biologically active compounds.
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Acknowledgments
This work was supported by the Slovak Research and Development Agency under contract No. APVV-19-0009 and by VEGA grant 2/0026/20 from the Slovak Academy of Sciences. The research leading to these results has received funding from the European Commission’s Seventh Framework Programme (FP7/2007-2013) under the grant agreement STREPSYNTH (project No. 613877). This work was co-funded by the Slovak Research and Development Agency under contract No. DO7RP-0037-12.
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Kormanec, J., Rezuchova, B., Novakova, R. (2021). Screening Systems for Stable Markerless Genomic Deletions/Integrations in Streptomyces Species. In: Barreiro, C., Barredo, JL. (eds) Antimicrobial Therapies. Methods in Molecular Biology, vol 2296. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1358-0_6
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DOI: https://doi.org/10.1007/978-1-0716-1358-0_6
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