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Actinomycetes genome engineering approaches

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Abstract

This review provides an overview of new technologies for DNA manipulations in actinomycetes exploiting recombinogenic engineering (Flp-FRT, Cre-loxP, Dre-rox, Tn5, GusA and I-SceI systems). We will describe some new vectors recently developed for engineering of complex phenotypes in actinomycetes. Several site-specific recombinases, transposons, reporter genes and I-SceI endonuclease have been utilized for genome manipulation in actinomycetes. Novel molecular tools will help to overcome many technical difficulties and will encourage new efforts to address the function of actinomycete genes.

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Acknowledgments

The work in the laboratory of AL was supported by the BMBF (GenBioCom), DFG (Lu1524/2-1) and ERC (EXPLOGEN) grants.

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Authors and Affiliations

  1. Albert-Ludwigs-University of Freiburg, Pharmaceutical Biologie and Biotechnology, Stefan-Meier-st. 19, 79104, Freiburg, Germany

    Theresa Siegl

  2. Helmholtz-Institute for Pharmaceutical Research Saarland, Campus, Building C2 3, 66123, Saarbrücken, Germany

    Andriy Luzhetskyy

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  1. Theresa Siegl
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Correspondence to Andriy Luzhetskyy.

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Siegl, T., Luzhetskyy, A. Actinomycetes genome engineering approaches. Antonie van Leeuwenhoek 102, 503–516 (2012). https://doi.org/10.1007/s10482-012-9795-y

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