Antimicrobial Therapies

1. Front Matter

   Pages i-xiii

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2. General Overview

   1. Front Matter

      Pages 1-1

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   2. Worldwide Clinical Demand for Antibiotics: Is It a Real Countdown?

      • Carlos Barreiro, José-Luis Barredo

      Pages 3-15

3. Antibiotic Screening

   1. Front Matter

      Pages 17-17

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   2. Collection, Extraction, and In Vitro Antibacterial Evaluation of Plants Used in Traditional Medicine

      • François Chassagne, Cassandra Leah Quave

      Pages 19-41

   3. Microbial Isolation and Characterization of New Antibiotic-Producing Strains from Decayed Wood

      • Óscar Velasco-Rodríguez, Mariana Fil, Laura García-Calvo, Katarina Kosalková, Carlos Barreiro

      Pages 43-57

   4. Characterizationharacterization of Extremophilic Actinomycetes Strains as Sources of Antimicrobial Agents

      • Diyora Abdukhakimova, Zhannur Markhametova, Saikal Shamkeeva, Ainur Zhulamanova, Lyudmila Trenozhnikova, Vladimir Berezin et al.

      Pages 59-75

   5. Dilution-to-Extinction Platform for the Isolation of Marine Bacteria-Producing Antitumor Compounds

      • Xulio Benítez, Jesús García, Elena G. Gonzalez, Fernando de la Calle

      Pages 77-87

4. Methodologies

   1. Front Matter

      Pages 89-89

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   2. Screening Systems for Stable Markerless Genomic Deletions/Integrations in Streptomyces Species

      • Jan Kormanec, Bronislava Rezuchova, Renata Novakova

      Pages 91-141

   3. Antibacterial Assay in a Screening Project

      • Francisco Romero, Antonio Fernández

      Pages 143-149

   4. Screening of Antibiotic Gene Clusters in Microorganisms Isolated from Wood

      • Mariana Fil, Óscar Velasco-Rodríguez, Laura García-Calvo, Alberto Sola-Landa, Carlos Barreiro

      Pages 151-165

   5. Methods in Screening Antiviral Drugs Against Enterovirus 71

      • Nadia Abubakar Omar Abubakar Bajaber, Babu Ramanathan

      Pages 167-184

   6. High-Efficiency Electroporation for Genetic Improvement of Fungal Strains

      • Jessica Cruz-Ramón, Francisco J. Fernández, Francisco Fierro

      Pages 185-194

   7. Penicillium chrysogenum Fermentation and Analysis of Benzylpenicillin by Bioassay and HPLC

      • Katarina Kosalková, Isabel-Clara Sánchez-Orejas, Laura Cueto, Carlos García-Estrada

      Pages 195-207

   8. Screening Fermentation and Extract Generation

      • Francisco Romero, Antonio Fernández

      Pages 209-216

   9. A Simple Antifungal assay for Testing Actinomycetes and Other Microbial Extracts

      • María A. Vinuesa, Antonio Fernández

      Pages 217-225

   10. A User Guide for the Identification of New RiPP Biosynthetic Gene Clusters Using a RiPPER-Based Workflow

       • Alaster D. Moffat, Javier Santos-Aberturas, Govind Chandra, Andrew W. Truman

       Pages 227-247Open Access

   11. Novel Methods to Identify Oxidative Stress-Producing Antibiotics

       • Álvaro Mourenza, José A. Gil, Luís M. Mateos, Michal Letek

       Pages 249-261

   12. Coupled Transcriptomics for Differential Expression Analysis and Determination of Transcription Start Sites: Design and Bioinformatics

       • Antonio Rodríguez-García, Alberto Sola-Landa, Rosario Pérez-Redondo

       Pages 263-278

Antimicrobial resistance will become a global health threat since antimicrobial treatments continue at the forefront of the defense against microbial infections. To respond to the issue, this detailed book explores vital methodologies currently in use to advance our understanding of antibiotic issues and answer the worldwide demand for novel antibiotics therapies. Beginning with a review chapter that guides the reader through the worldwide demand for novel antibiotics therapies, the volume continues with sections covering new screening procedures and environmental sources, advances in analytical, microbiological, and biotechnological methodologies, antibiotic production and antibiotic resistances, as well as considerations of drug trials and clinical concerns regarding multi-resistant patients. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Antimicrobial Therapies: Methods and Protocols provides a reference source for health, laboratory, and industrial professionals, as well as for graduate students in a number of bio-sanitary disciplines, including medicine, nursery, biotechnology, veterinary, microbiology, genetics, molecular biology, nutrition, farming, and more.

Chapter “14” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

• Antibiotics
• Antimicrobial resistance
• Microbial infections
• Multidrug-resistant pathogens
• Antibacterials

• INBIOTEC (Instituto de Biotecnología de León), Parque Científico de León, León, Spain

  Carlos Barreiro

• Department of Biotechnology, Crystal Pharma A Subsidiary of Albany Molecular Research Inc. (AMRI) Parque Tecnológico de León, León, Spain

  José-Luis Barredo

Policies and ethics