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In Vitro Apoptotic Cell Death Assessment

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Toxicity Assessment

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2240))

Abstract

Chemical compounds induce cytotoxicity by various mechanisms, including interference in membrane integrity, metabolism, cellular component degradation or release, and cell division. Between the classic death pathways, namely, autophagy, apoptosis, and necrosis, apoptosis have been in the focus for the last several years as an important pathway for the toxicity of different types of xenobiotics. Because of that, having the tools to evaluate it is key for understanding and explaining the toxicodynamics of different classes of substances. Here, we describe a wide array of classic assays that can be easily implemented to evaluate apoptosis induction.

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Author information

Authors and Affiliations

  1. Department of Clinical, Toxicological and Bromatological Analysis, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

    Lilian Cristina Pereira & Raul Ghiraldelli Miranda

  2. Department of Bioprocesses and Biotechnology, Faculty of Agronomic Sciences of Botucatu, São Paulo State University, Botucatu, SP, Brazil

    Lilian Cristina Pereira

  3. Center for Evaluation of Environmental Impact on Human Health (TOXICAM), Botucatu, São Paulo, Brazil

    Lilian Cristina Pereira

  4. Federal Institute of Science and Technology Education of Rondônia—Campus Porto Velho Calama, Porto Velho, RO, Brazil

    Alecsandra Oliveira de Souza

  5. FFCLRP-USP, Department of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil

    Alecsandra Oliveira de Souza & Daniel Junqueira Dorta

  6. Instituto Nacional de Tecnologias Alternativas de Detecção, Avaliação Toxicológica e Remoção de Micropututantes e Radioativos (INCT-DATREM), Unesp, Instituto de Química, Caixa Postal 355, CEP: 14800-900, Araraquara, SP, Brazil

    Daniel Junqueira Dorta

Authors
  1. Lilian Cristina Pereira
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  2. Alecsandra Oliveira de Souza
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  3. Raul Ghiraldelli Miranda
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  4. Daniel Junqueira Dorta
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Corresponding author

Correspondence to Daniel Junqueira Dorta .

Editor information

Editors and Affiliations

  1. MitoLab, Department of Life Sciences, Center for Neurosciences and Cell Biology of the University of Coimbra, Coimbra, Portugal

    Carlos Manuel Marques Palmeira

  2. School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil

    Danielle Palma de Oliveira

  3. Department of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil

    Daniel Junqueira Dorta

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Pereira, L.C., de Souza, A.O., Miranda, R.G., Dorta, D.J. (2021). In Vitro Apoptotic Cell Death Assessment. In: Palmeira, C.M.M., de Oliveira, D.P., Dorta, D.J. (eds) Toxicity Assessment. Methods in Molecular Biology, vol 2240. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1091-6_17

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  • DOI: https://doi.org/10.1007/978-1-0716-1091-6_17

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1090-9

  • Online ISBN: 978-1-0716-1091-6

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