Abstract
A misconception regarding the sensitivity of nonradioactive methods for screening genomic DNA libraries often hinders the establishment of these environmentally friendly techniques in molecular biology laboratories. Nonradioactive probes, properly prepared and quantified, can detect DNA target molecules to the femtomole range. However, appropriate hybridization techniques and detection methods should also be adopted for an efficient use of nonradioactive techniques. Detailed descriptions of genomic library handling before and during the nonradioactive hybridization and detection are often omitted from publications. This chapter aims to fill this void by providing a collection of technical tips on hybridization and detection techniques.
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References
Ausubel, F. M., Brent, R., Kingston, R. E., eds. (1994) Current Protocols in Molecular Biology. John Wiley and Sons, New York.
Sambrook, J. and Russell, D. W. (2001) Molecular Cloning: A Laboratory Manual. 3 ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Reid, A. (2002) Target format and hybridization conditions, in Gene Probes, Principles and Protocols. (Aquino de Muro, M. and Rapley, R. eds.), Humana, Totowa, NJ, pp. 1–11.
Dubitsky, A. (1997) Blocking strategies for nylon membranes used in enzyme-linked immunosorbent assays. IVDT (July), 53.
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© 2007 Humana Press Inc.
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Hilario, E. (2007). Overview of Hybridization and Detection Techniques. In: Hilario, E., Mackay, J. (eds) Protocols for Nucleic Acid Analysis by Nonradioactive Probes. Methods in Molecular Biology, vol 353. Humana Press. https://doi.org/10.1385/1-59745-229-7:27
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DOI: https://doi.org/10.1385/1-59745-229-7:27
Publisher Name: Humana Press
Print ISBN: 978-1-58829-430-2
Online ISBN: 978-1-59745-229-8
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