Editors:
- Readily reproducible protocols for the analysis of nucleic acids by nonradioactive methods
- New fluorescent techniques such as Real Time PCR and microarrays
- Follow step-by-step instructions that ensure successful results
- Master tricks of the trade, troubleshoot, and avoid known pitfalls
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 353)
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Table of contents (19 protocols)
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Front Matter
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Nucleic Acid Extractions
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Front Matter
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Indirect Detection
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Front Matter
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Fluorescent Labeling and Detection
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Front Matter
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Kinetic (“Real-Time”) PCR
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Front Matter
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About this book
The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Reviews
From the reviews of the second edition:
...this book provides a collection of good protocols which are in general clearly written. They can be easily employed on the bench without being a specialist in a particular fields. - FEBS Letters
...Covers the basics of a variety of probe-labelling systems very well...a very interesting read. It could save you money and trouble from the radiation safety officer.-Biotechnology and Applied Biochemistry
"This is a set of protocols for various types of nucleic acid analysis using nonradiocative methods. … The goal is to provide protocols for nucleic acid analysis using other than radioactive reporter systems. This approach is commonplace at present, so a volume of practical utility has merit. … The target audience would be research laboratory personnel working in this general area. The editors have recruited a group of contributors with worldwide representation." (Eugene A Davidson, Doody’s Review Service, November, 2007)
"The editors of this volume have gathered together a variety of protocols for anyone who wishes to use nonradioactive methods for the analysis of their chosen nucleic acid molecules. ... Combined with the excellent overviews on microarrays as well as hybridization and detection techniques, and several useful assays for the application of these techniques for nucleic acid analysis, scientists will find this book handy for their research and educators will adopt it as a useful teaching tool for advanced students." (Tzvi Tzfira, The Quarterly Review of Biology, Vol. 83, 2008)
Editors and Affiliations
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Gene Technologies, HortResearch Ltd., Mt. Albert, Auckland, New Zealand
Elena Hilario
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Applied Science, Roche Diagnostics, Auckland, New Zealand
John Mackay
Bibliographic Information
Book Title: Protocols for Nucleic Acid Analysis by Nonradioactive Probes
Editors: Elena Hilario, John Mackay
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1385/1597452297
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2007
Hardcover ISBN: 978-1-58829-430-2Published: 12 January 2007
Softcover ISBN: 978-1-61737-564-4Published: 01 December 2010
eBook ISBN: 978-1-59745-229-8Published: 02 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: 321
Number of Illustrations: 61 b/w illustrations, 5 illustrations in colour
Topics: Biochemistry, general, Human Genetics, Cell Biology, Plant Sciences