Abstract
Although rose transformation is successful, it remains difficult to transform myriad rose species as well as different rose genotypes. In this protocol, a detailed description of rose transformation is presented. This protocol relied on Agrobacterium-mediated transfer of embryogenic callus cultures. There are many critical steps that must be followed to achieve successful transformation; however, it is important to keep in mind that these apply to a selected number of genotypes, and as a different genotype is subjected to transformation, modifications of this protocol must be made to achieve successful transformation.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Mastalerz, J.W. and Langhans, R.W. (1969) Rose, a manual on the culture, management, disease, insects, economics and breeding of greenhouse roses. New York State Flower Growers Association, New York, NY.
Rajapakse, S., Hubbard, M., Kelly, J.W., Abbott, A.G., and Ballard, R.E. (1992) Identification of rose cultivars by restriction fragment length polymorphism. Scientia Hort. 52, 237–245.
Vainstein, A. and Ben-Meir, H. (1994) DNA fingerprint analysis of roses. J. Amer. Soc. Hort. Sci. 119, 1099–1103.
De Wit, J.C., Esendam, H.F., Honkanen, J.J., and Tuominen, U. (1990) Somatic embryogenesis and regeneration of flowering plants in rose. Plant Cell Rep. 9, 456–458.
Arene, L., Pellegrino, C., and Gudin, S. (1993) A comparison of the somaclonal variation level of Rosa hybrida L. cv. Meirutral plants regenerated from callus of direct induction from different vegetative and embryonic tissues. Euphytica 71, 83–90.
van der Salm, T.P.M., van der torn, C.J.G., Hanischten Cate, C.H., and Dons, H.J.M. (1996) Somatic embryogenesis and shoot regeneration from excised adventitious roots of the rootstock Rosa hybrida cv. Money Way. Plant Cell Rep. 15, 522–526.
Hsia, C. and Korban, S.S. (1996) Organogenesis and somatic embryogenesis in callus cultures of Rosa hybrida and Rosa chinensis minima. Plant Cell Tiss. Org. Cult. 44, 1–6.
Kintzois, S., Manos, C., and Makri, O. (1999) Somatic embryogenesis from mature leaves of rose (Rosa sp.). Plant Cell Rep. 18, 467–472.
Li, X., Krasnyanski, S., and Korban, S.S. (2002) Somatic embryogenesis, secondary somatic embryogenesis, and shoot organogenesis in Rosa. J. Plant Physiol. 159, 313–319.
Kunitake, H., Imamizo, H., and Mii, M. (1993) Somatic embryogenesis and plant regeneration from immature seed-derived calli of rugosa rose (Rosa rugosa Thumb). Plant Sci. 90, 187–194.
Visessuwan, R., Kawai, T., and Mii, M. (1997) Plant regeneration systems from leaf segment culture through embryogenic callus formation of Rosa hybrida and R. canina. Breed. Sci. 47, 217–222.
Firoozabady, E., Moy, Y., Courtney-Gutterson, N., and Robinson, K. (1994) Regeneration of transgenic rose (Rosa hybrida) plants from embryogenic tissue. Nat. Biotechnol. 12, 609–613.
van der Salm, T.P.M., Bouwer, R., van Dijk, A.J., et al. (1997) Production of rol gene transformed plants of Rosa hybrida L. and characterization of their rooting ability. Mol. Breeding 3, 39–47.
van der Salm, T.P.M., Bouwer, R., van Dijk, A.J., et al. (1998) Stimulation of scion bud release by rol gene transformed rootstocks of Rosa hybrida L. J. Exp. Bot. 49, 847–852.
Marchant, R., Power, J.B., Lucas, J.A., and Davey, M.R. (1998) Biolistic transformation of rose (Rosa hybrida L.). Ann. Bot. 81, 109–114.
Marchant, R., Davey, M.R., Lucas, J.A., Lamb, C.J., Dixon, R.A., and Power, J.B. (1998) Expression of a chitinase in rose (Rosa hybrida L) reduces development of black spot disease (Diplocarpon rosae Wolf). Mol. Breed. 4, 187–194.
Li, X., Krasnyanski, S., and Korban S.S. (2002) Optimization of the uidA gene transfer into somatic embryos of rose via Agrobacterium tumefaciens. Plant Physiol. Biochem. 40, 453–459.
Li, X., Gasic, K., Cammue, B., Broekaert, W., and Korban, S.S. (2003) Transgenic rose lines harboring an antimicrobial protein gene, Ace-AMP1, demonstrate enhanced resistance to powdery mildew (Sphaerotheca pannosa). Planta 218, 226–232.
Kim, C.K., Chung, J.D., Park, S.H., Burrell, A.M., Kamo, K.K., and Byrne, D.H. (2004) Agrobacterium tumefaciens-mediated transformation of Rosa hybrida using the green fluorescent protein (GFP) gene. Plant Cell Tiss. Org. Cult. 78, 107–111.
Hsia, C. and Korban, S.S. (1996) Factors affecting in vitro establishment and shoot proliferation of Rosa hybrida L and Rosa chinensis minima. In Vitro Cell Dev. Biol.-Plant 32, 217–222.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2006 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Korban, S.S., Gasic, K., Li, X. (2006). Rose (Rosa hybrida L.). In: Wang, K. (eds) Agrobacterium Protocols Volume 2. Methods in Molecular Biology, vol 344. Humana Press. https://doi.org/10.1385/1-59745-131-2:351
Download citation
DOI: https://doi.org/10.1385/1-59745-131-2:351
Publisher Name: Humana Press
Print ISBN: 978-1-58829-843-0
Online ISBN: 978-1-59745-131-4
eBook Packages: Springer Protocols