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Transgenic rose lines harboring an antimicrobial protein gene, Ace-AMP1, demonstrate enhanced resistance to powdery mildew (Sphaerotheca pannosa)

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Abstract

An antimicrobial protein gene, Ace-AMP1, was introduced into Rosa hybrida cv. Carefree Beauty via Agrobacterium-mediated transformation. A total of 500 putative transgenic plants were obtained from 100 primary embryogenic calli co-cultivated with A. tumefaciens following selection on a regeneration medium containing 100 mg/l kanamycin. Polymerase chain reaction analysis of these putative transgenic lines, using primers for both Ace-AMP1 and neomycin phosphotransferase (npt II) genes, showed that 62% of these plants were positive for both transgenes. These lines were further confirmed for stable integration of Ace-AMP1 and npt II genes by Southern blotting. Transcription of the Ace-AMP1 transgene in various transgenic rose lines was determined using Northern blotting. Transgenic rose lines inoculated with conidial spores of Sphaerotheca pannosa (Wallr.: Fr.) Lev. var. rosae showed enhanced resistance to powdery mildew using both a detached-leaf assay and an in vivo greenhouse whole-plant assay.

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Fig. 1. a
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Fig. 5a, b.
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Abbreviations

AMP:

antimicrobial protein

npt II :

neomycin phosphotransferase

PGR:

plant growth regulator

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Acknowledgement

This project was supported by a grant received from the Gloeckner Foundation.

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Authors and Affiliations

  1. Department of Natural Resources and Environmental Sciences, 310 ERML, University of Illinois, 1201 W. Gregory, Urbana, IL 618001, USA

    Xiangqian Li, Ksenjia Gasic & Schuyler S. Korban

  2. Centre voor Microbiële en Plantengenetica, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, 3001, Heverlee, Belgium

    Bruno Cammue & Willem Broekaert

Authors
  1. Xiangqian Li
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  2. Ksenjia Gasic
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  3. Bruno Cammue
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  4. Willem Broekaert
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  5. Schuyler S. Korban
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Correspondence to Schuyler S. Korban.

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Li, X., Gasic, K., Cammue, B. et al. Transgenic rose lines harboring an antimicrobial protein gene, Ace-AMP1, demonstrate enhanced resistance to powdery mildew (Sphaerotheca pannosa). Planta 218, 226–232 (2003). https://doi.org/10.1007/s00425-003-1093-5

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  • DOI: https://doi.org/10.1007/s00425-003-1093-5

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