Skip to main content
SpringerLink
Log in

Amplification of Lectin-Gold Histochemistry

  • Protocol
Lectin Methods and Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 9))

  • 1795 Accesses

Abstract

Colloidal gold was developed as a probe for microscopy in the late 1970s. However, Roberts (1) had previously injected gold salts in living animals and detected the tissue-gold interactions microscopically by a photochemical method. With this method, metallic silver was deposited around gold particles Roberts based his method on the idea of Liesegang and Rieder (2), who visualized silver compounds injected into animals by a photographic process.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Roberts, W J. (1935) A new method for the detection of gold in animal tissues physical development. Proc R Acad Sci Amsterdam 38, 540–544.

    CAS  Google Scholar 

  2. Liesegang, R E and Rieder, W (1921) Versuche mit einer «Keim-methode« zum Nachweis von Silber in Gewebsschnitten Z Wissench Mikrosk 38, 334–338

    Google Scholar 

  3. Danscher, G. (1981) Histochemical demonstration of heavy metals A revised version of the sulphide silver method suitable for light and electronmicroscopy Histochemistry 71, 1–16

    Article  PubMed  CAS  Google Scholar 

  4. Danscher, G (1981) Localization of gold in biological tissue. A photochemical method for light and electron microscopy Histochemistry 71, 81–88

    Article  PubMed  CAS  Google Scholar 

  5. Holgate, C S, Jackson, P, Cowen, P N, and Bird, C C (1983) Immunogold-silver staining: new method of immunostaining with enhanced sensitivity J Histochem Cytochem 31, 938–944.

    PubMed  CAS  Google Scholar 

  6. Faulk, W. P and Taylor, G M (1971) An immnunocolloidal method for the electron microscopy Immunochemistry 8, 1081–1083

    Article  PubMed  CAS  Google Scholar 

  7. Springall, D. R, Hacker, G W., Grimelius, L, and Polak, J M (1984) The potential of the immunogold silver staining for paraffin sections Histochemistry 81, 603–608.

    Article  PubMed  CAS  Google Scholar 

  8. Hacker, G. W., Grimelius, L, Danscher, G, Bernatzky, G, Muss, W, Adam, H, and Thurner, J (1988) Silver acetate autometallography an alternative enhancement technique for immunogold-silver staining (IGSS) and silver amplification of gold, silver, mercury and zinc in tissues J Histotechnol 11, 213–221.

    CAS  Google Scholar 

  9. Taatjes, D. J., Schaub, U, and Roth, J. (1987) Light microscopical detection of antigens and lectin binding sites with gold-labelled reagents on semithin Lowicryl K4M sections. usefulness of the photochemical silver reaction for signal amplification Histochem J 19, 235–245

    Article  PubMed  CAS  Google Scholar 

  10. Krenácks, T., Lászik, Z., and Dobó, E (1989) Application of immunogold-silver staining and immunoenzymatic methods in multiple labelling of human pancreatic Langerhans islet cell Acta Histochem 85, 79–85

    Google Scholar 

  11. Skutelsky, E, Goyal, V., and Alroy, J (1987) The use of avidin-gold complex for light microscopic localization of lectin receptors Histochemistry 86, 291–295

    Article  PubMed  CAS  Google Scholar 

  12. Madrid, J. F, Castells, M T., Martínez-Menárguez, J A, Avilés, M, Hernández, F, and Ballesta, J. (1994) Subcellular characterization of glycoproteins in the principal cells of human gallbladder. A lectin cytochemical study. Histochemistry 101, 195–204

    Article  PubMed  CAS  Google Scholar 

  13. Scopsi, L and Larsson, L-I (1985) Increased sensitivity in immunocytochemistry Effects of double application of antibodies and of silver intensification on immnunogold and peroxidase-antiperoxidase staining techniques Histochemistry 82,321–329

    Article  PubMed  CAS  Google Scholar 

  14. Kehle, T. and Herzog, V (1990) Quantification of colloidal gold by electron microscopy, in Colloidal Golds Principles, Method, and Application, vol 3 (Hayat, M. A, ed), Academic San Diego, CA, pp 117–137

    Google Scholar 

  15. Yokata, S (1988) Effect of particle size on labeling density for catalase in protein A-gold immunocytochemistry J Histochem Cytochem 36, 107–119

    Google Scholar 

  16. Roth, J, Lucocq, J M, and Charest, P M (1984) Light and electron microscopic demonstration of sialic acid residues with the lectin from Limax flavus a cytochemical affinity technique with the use of fetuin-gold complexes J Histochem Cytochem 32, 1167–1177

    PubMed  CAS  Google Scholar 

  17. Roth, J (1986) Post-embedding cytochemistry with gold labeled reagent a review J Microsc 143, 125–137

    PubMed  CAS  Google Scholar 

  18. Sibon, O C M, Humbel, B, De Graaf, A., Verkleij, A J, and Cremers, F F M (1994) Ultrastructural localization of epidermal growth factor (EGF)-receptor transcripts in the cell nucleus using pre-embedding in situ hybridization in combination with ultra-small gold probes and silver enhancement Histochemistry 101, 223–232

    Article  PubMed  CAS  Google Scholar 

  19. Scopsi, L, Larsson, L-I, Bastholm, L, and Nielsen, M H (1986) Silver-enhanced colloidal gold probes as markers for scanning electron microscopy Histochemistry 86, 35–41.

    Article  PubMed  CAS  Google Scholar 

  20. Lackie, P. M, Hennessy, R J, Hacker, G W, and Polak, J M (1985) Investigation of immunogold-silver staining by electron microscopy Histochemistry 83, 545–550

    Article  PubMed  CAS  Google Scholar 

  21. van den Pol, A N (1986) Tyrosine hydroxylase immumoreactive neurons throughout the hypothalamus receive glutamate decarboxylase immunoreactive synapses a double preembedding immunocytochemical study with particulate silver and HRP J Neurosci 6, 877–897.

    PubMed  Google Scholar 

  22. Walther, P and Muller, M (1985) Detection of small (5–15 nm) gold-labelled surface antigens using backscattered electrons, in The Science of Biologica1 Specimen Preparation for Microscopy and Microanalysis (Muller, M, Becker, R P., Bodye, A., and Wolosweick, J J, eds.), SEM, Chicago, pp 195–201

    Google Scholar 

  23. Herter, P., Laube, G, Gronczewski, J, and Minuth, W W. (1993) Silver-enhanced colloidal-gold of rabbit kidney collecting-duct cell surfaces imaged by scanning electron microscopy J Microsc 171, 107–115

    PubMed  CAS  Google Scholar 

  24. Namork, E. and Heier, H E (1989) Silver enhancement of gold probes (5–40 nm) single and double labeling of antigenic sites on cell surface imaged with backscattered electrons J Electr Microsc Tech 11, 102–108

    Article  CAS  Google Scholar 

  25. Scopsi, L (1989) Silver-enhanced colloidal gold method, in Colloidal Golds Principles, Method, and Application, vol 1 (Hayat, M A, ed), Academic, San Diego, pp 251–295

    Google Scholar 

  26. Burry, R. W, Vandré, D. D, and Hayes, D M (1992) Silver enhancement of gold antibody probes in pre-embedding electron microscopic immunocytochemistry J Histochem Cytochem 40, 1849–1856

    PubMed  CAS  Google Scholar 

  27. Shimizu, H, Masunaga, T, Ishiko, K, Hashimoto, T, Garrod, D R, Shida, H, and Nishikawa, T (1994) Demonstration of desmosomal antigen by electron microscopy using cryofixed and cryosubstituted skin with silver-enhanced gold probe J Histochem Cytochem 42, 687–462

    PubMed  CAS  Google Scholar 

  28. Liesegang, R (1928) Histologische Versilberung Z Wiss Mikr 45,273–279

    Google Scholar 

  29. Grimelius, L (1968) A silver nitrate stain for A2 cells of human pancreatic islets Acta Soc Med Ups 73, 243

    PubMed  CAS  Google Scholar 

  30. Hacker, G. W (1989) Sliver-enhanced colloidal gold for light microscopy, in Colloidal Golds Principles, Method, and Application, vol 1 (Hayat, M A, ed), Academic, San Diego, pp 297–321

    Google Scholar 

  31. Fujimori, O and Nakamura, M (1985) Protein A gold-silver staining method for light microscopic immunohistochemistry Arch Histol Jpn 48, 449–459

    Article  PubMed  CAS  Google Scholar 

  32. DiFiglia, C J and Fields, K. L. (1987) A comparison of indirect immunofluorescence and immunogold-silver staining for surface antigens of PNS and CNS cells J Neuroimmunol 16, 43–49

    Article  Google Scholar 

  33. Geuze, H S, Slot, J W, Yanagibashi, K, McCraken, J A, Schwartz, A L, and Hall, P F. (1987) Immunogold cytochemistry of cytochrome P-450 in porcine adrenal cortex Histochemistry 86, 551–561

    Article  PubMed  CAS  Google Scholar 

  34. Goode, D and Maugel, T K (1987) Backscattered electron imaging of immuno-gold-labelled and silver-enhanced microtubules in mammalian cells J Electron Microsc Tech 5, 263–273

    Article  Google Scholar 

  35. Danscher, G (1983) A silver method for counterstaining plastic embedded tissue Stain Technol 58, 365–372

    PubMed  CAS  Google Scholar 

  36. Lageman, A and Buccheim, P (1985) Die Gold-Silber-Technik-ein neues immunohistochemisches Verfahren zum Nachweis von Zellmembranantigenen Acta Histochem 76,113–123

    PubMed  CAS  Google Scholar 

  37. Lah, J J, Hayes, D M., and Burry, R. W (1990) A neutral pH silver development method for the visualization of 1-nanometer gold particles in pre-embedding electron macroscopic immunocytochemistry J Histochem Cytochem 38,503–513

    PubMed  CAS  Google Scholar 

  38. Danscher, G (1982) Exogenous selenium in the brain A histochemical technique for light and electron microscopical localization of catalytic selenium bonds Histochemistry 76, 281–293

    Article  PubMed  CAS  Google Scholar 

  39. De Waele, M (1989) Silver-enhanced colloidal gold for the detection of leukocyte cell surface antigens in dark-field and epipolarization microscopy, in Colloidal Golds Principles, Method, and Application, vol. 2. (Hayat, M A, ed), Academic, San Diego, pp 443–467

    Google Scholar 

  40. Manigley, C and Roth, J. (1985) Applications of immunocolloids in light microscopy. IV. Use of photochemical silver staining in a simple and efficient double-staining technique J Histochem Cytochem 33, 1247–1251

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Cell Biology and Morphological Sciences, School of Medicine and Dentistry, University of the Basque Country, Leioa, Vizcaya, Spain

    Juan F. Madrld

  2. Department of Cell Biology, School of Medicine, University of Murcia, Spain

    Francisco Hernández & José Ballesta

Authors
  1. Juan F. Madrld
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Francisco Hernández
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. José Ballesta
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of Liverpool, UK

    Jonathan M. Rhodes  & Jeremy D. Milton  & 

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Humana Press Inc.

About this protocol

Cite this protocol

Madrld, J.F., Hernández, F., Ballesta, J. (1998). Amplification of Lectin-Gold Histochemistry. In: Rhodes, J.M., Milton, J.D. (eds) Lectin Methods and Protocols. Methods in Molecular Medicine™, vol 9. Humana Press. https://doi.org/10.1385/0-89603-396-1:121

Download citation

  • DOI: https://doi.org/10.1385/0-89603-396-1:121

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-396-2

  • Online ISBN: 978-1-59259-593-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation