Abstract
Single-strand breaks are among the most prevalent lesions found in DNA. Traditional electrophoretic methods (e.g., the Comet assay) used for investigating these lesions rely on alkaline conditions to denature DNA prior to electrophoresis. However, the presence of alkali-labile sites in DNA can result in the introduction of additional single-strand breaks upon alkali treatment during DNA sample processing. Herein, we describe a neutral glyoxal gel electrophoresis assay which is based on alkali-free DNA denaturation and is suitable for qualitative and semi-quantitative analyses of single-strand breaks in DNA isolated from different organisms.
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Acknowledgements
This work was supported in part by National Institute of Environmental Health Science grants (P42-ES05948, P30-ES10126).
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Pachkowski, B., Nakamura, J. (2013). A Neutral Glyoxal Gel Electrophoresis Method for the Detection and Semi-quantitation of DNA Single-Strand Breaks. In: Makovets, S. (eds) DNA Electrophoresis. Methods in Molecular Biology, vol 1054. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-565-1_8
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DOI: https://doi.org/10.1007/978-1-62703-565-1_8
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Publisher Name: Humana Press, Totowa, NJ
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