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The Prospective Isolation of Viable, High Ploidy Megakaryocytes from Adult Murine Bone Marrow by Fluorescence Activated Cell Sorting

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Stem Cell Niche

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1035))

Abstract

Mature megakaryocytes (MM) can be up to 65 μM in diameter and due to their size, viable and pure MM populations have been difficult to isolate in large numbers. Here in, we report a fluorescence activated cell sorting (FACS) method by which viable and pure populations of 8 N, 16 N, 32 N, and 64 N MM can be isolated from murine bone marrow (BM).

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Acknowledgments

The authors thank Dani Cardozo for assistance with animal work, Michael Reitsma and Andrew Fryga for intellectual input and flow cytometric support. In addition, we also thank Kathryn Flanagan and Karen Clarke for flow cytometric support.

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Authors and Affiliations

  1. Materials Science and Engineering, Commonwealth Scientific and Industrial Research Organization, Melbourne, VIC, Australia

    Shen Y. Heazlewood

  2. Materials Science and Engineering, Commonwealth Scientific and Industrial Research Organization, Melbourne, Australia

    Brenda Williams, Melonie J. Storan & Susan K. Nilsson

  3. Department of Anatomy and Developmental Biology, Monash University, Melbourne, Australia

    Susan K. Nilsson

Authors
  1. Shen Y. Heazlewood
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  2. Brenda Williams
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  3. Melonie J. Storan
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  4. Susan K. Nilsson
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Editor information

Editors and Affiliations

  1. Regenerative Medicine Program, Ottawa Hospital Research Institute Sprott Centre for Stem Cell Research, Ottawa, Canada

    Kursad Turksen

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Heazlewood, S.Y., Williams, B., Storan, M.J., Nilsson, S.K. (2013). The Prospective Isolation of Viable, High Ploidy Megakaryocytes from Adult Murine Bone Marrow by Fluorescence Activated Cell Sorting. In: Turksen, K. (eds) Stem Cell Niche. Methods in Molecular Biology, vol 1035. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-508-8_10

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  • DOI: https://doi.org/10.1007/978-1-62703-508-8_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-507-1

  • Online ISBN: 978-1-62703-508-8

  • eBook Packages: Springer Protocols

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