Abstract
Protein–protein interaction is an essential biochemical event that mediates various cellular processes including gene expression, intracellular signaling, and intercellular interaction. Understanding such interaction is key to the elucidation of mechanisms of cellular processes in biology and diseases. The hypoxia-inducible transcription factor HIF-1α possesses a non-transcriptional activity that competes with c-Myc for Sp1 binding, whereas its isoform HIF-2α lacks Sp1-binding activity due to phosphorylation. Here, we describe the use of in vitro translation to effectively investigate the dynamics of protein–protein interactions among HIF-1α, c-Myc, and Sp1 and to demonstrate protein phosphorylation as a molecular determinant that functionally distinguishes HIF-2α from HIF-1α.
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Acknowledgment
This work was supported in part by the Public Health Service grant CA-131355 from the National Cancer Institute.
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To, K.KW., Huang, L.E. (2013). An Efficient Way of Studying Protein–Protein Interactions Involving HIF-α, c-Myc, and Sp1. In: Soucek, L., Sodir, N. (eds) The Myc Gene. Methods in Molecular Biology, vol 1012. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-429-6_6
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DOI: https://doi.org/10.1007/978-1-62703-429-6_6
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-428-9
Online ISBN: 978-1-62703-429-6
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