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  • © 2013

The Myc Gene

Methods and Protocols

  • Presents current up-to-date methodologies
  • Features tips on trouble-shooting and avoiding known pitfalls
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1012)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
  2. The Myc World Within Reach

    • Nicole M. Sodir, Laura Soucek
    Pages 1-6
  3. Methods for the Expression, Purification, Preparation, and Biophysical Characterization of Constructs of the c-Myc and Max b-HLH-LZs

    • Marie-Eve Beaulieu, FranƧois-Olivier McDuff, MikaĆ«l BĆ©dard, Martin Montagne, Pierre Lavigne
    Pages 7-20
  4. Analyzing Myc in Cell Transformation and Evolution

    • Markus Hartl, Klaus Bister
    Pages 21-49
  5. Identifying Myc Interactors

    • Romina Ponzielli, William B. Tu, Igor Jurisica, Linda Z. Penn
    Pages 51-64
  6. Methods for Determining Myc-Induced Apoptosis

    • Dan Lu, Trevor D. Littlewood
    Pages 85-98
  7. Methods to Study MYC-Regulated Cellular Senescence

    • Vedrana Tabor, Matteo Bocci, Lars-Gunnar Larsson
    Pages 99-116
  8. Chromatin Immunoprecipitation Assays for Myc and N-Myc

    • Bonnie L. Barrilleaux, Rebecca Cotterman, Paul S. Knoepfler
    Pages 117-133
  9. Investigating Myc-Dependent Translational Regulation in Normal and Cancer Cells

    • John T. Cunningham, Michael Pourdehnad, Craig R. Stumpf, Davide Ruggero
    Pages 201-212
  10. Studying Mycā€™s Role in Metabolism Regulation

    • Anne Le, Chi V. Dang
    Pages 213-219
  11. Methods to Assess Myc Function in Intestinal Homeostasis, Regeneration, and Tumorigenesis

    • David J. Huels, Patrizia Cammareri, Rachel A. Ridgway, Jan P. Medema, Owen J. Sansom
    Pages 237-248
  12. Cell-Based Methods for the Identification of MYC-Inhibitory Small Molecules

    • Catherine A. Burkhart, Michelle Haber, Murray D. Norris, Andrei V. Gudkov, Mikhail A. Nikiforov
    Pages 255-264
  13. Back Matter

    Pages 265-282

About this book

Myc controls multiple cellular functions, including cell proliferation, growth, differentiation and death, both directly and indirectly, through its modulation of downstream transcriptional programs. In The Myc Gene: Methods and Protocols, experts in the field summarize the standard and novel techniques that allow the studying of Myc mechanism of action in normal and cancer cells, in vitro and in vivo, in one succinct manual. Written in the highly successful Methods in Molecular Biology Ā series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Editors and Affiliations

  • FundaciĆ³ Vall d'Hebron Institute of Onco, Barcelona, Spain

    Laura Soucek

  • Department of Biochemistry Sanger Building, University of Cambridge, Cambridge, United Kingdom

    Nicole M. Sodir

Bibliographic Information

  • Book Title: The Myc Gene

  • Book Subtitle: Methods and Protocols

  • Editors: Laura Soucek, Nicole M. Sodir

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-429-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-428-9Published: 05 September 2013

  • Softcover ISBN: 978-1-4939-6017-0Published: 23 August 2016

  • eBook ISBN: 978-1-62703-429-6Published: 05 September 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 282

  • Number of Illustrations: 15 b/w illustrations, 29 illustrations in colour

  • Topics: Cell Biology, Human Genetics

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access