Abstract
Transfection of mammalian cells has long been an extremely powerful approach for the study of the effects of specific gene expression on cell function. Until recently, however, this approach has been unavailable for the study of gene function in adult cardiac myocytes. Here, an adaptation of the biolistic method to the transfection of adult cardiac myocytes is described. DNA is precipitated onto gold particles in the absence of PVP and the particles are biolistically delivered to freshly isolated adult rat cardiomyocytes via a Bio-Rad Helios System gene gun. The myocytes are cultured in the absence of bovine serum albumin and expression of the introduced genes, in phenotypically intact myocytes, is robust within 12–24 h.
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References
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Acknowledgments
This work was supported by funding to D.F. from the Canadian Institutes for Health Research and the Heart and Stroke Foundation of British Columbia and the Yukon.
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© 2013 Springer Science+Business Media, LLC
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Steele, D.F., Dou, Y., Fedida, D. (2013). Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes. In: Sudowe, S., Reske-Kunz, A. (eds) Biolistic DNA Delivery. Methods in Molecular Biology, vol 940. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-110-3_12
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DOI: https://doi.org/10.1007/978-1-62703-110-3_12
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-109-7
Online ISBN: 978-1-62703-110-3
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