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Biolistic DNA Delivery

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Stephan Sudowe

    1. Ganzimmun Diagnostics AG, Mainz, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Angelika B. Reske-Kunz

    1. University Medical Center, Department of Dermatology, Johannes Gutenberg-University, Mainz, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Covers biolistic gene transfer into plants, nematodes, or in vitro and in vivo mammalian cells
  • Includes lab-ready protocols with clear step-by-step direction from leading researchers in the field
  • Features key tips and implementation advice to guarantee successful results

Part of the book series: Methods in Molecular Biology (MIMB, volume 940)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
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  2. Biolistic DNA Delivery in Plant Science and Agricultural Biotechnology

    1. Front Matter

      Pages 1-1
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    2. Comparison Between Agrobacterium-Mediated and Direct Gene Transfer Using the Gene Gun

      • Caixia Gao, Klaus K. Nielsen
      Pages 3-16

    3. Transient Gene Expression in Epidermal Cells of Plant Leaves by Biolistic DNA Delivery

      • Shoko Ueki, Shimpei Magori, Benoît Lacroix, Vitaly Citovsky
      Pages 17-26

    4. Transformation of Nuclear DNA in Meristematic and Embryogenic Tissues

      • Mulpuri Sujatha, K. B. R. S. Visarada
      Pages 27-44

    5. Biolistic DNA Delivery to Leaf Tissue of Plants with the Non-vacuum Gene Gun (HandyGun)

      • Anssi L. Vuorinen, Arto Nieminen, Victor Gaba, Sidona Sikorskaite, Jari P. T. Valkonen
      Pages 45-51

    6. HandGun-Mediated Inoculation of Plants with Viral Pathogens for Mechanistic Studies

      • Victor Gaba, Moshe Lapidot, Amit Gal-On
      Pages 53-62

    7. Biolistics-Based Gene Silencing in Plants Using a Modified Particle Inflow Gun

      • Kevin M. Davies, Simon C. Deroles, Murray R. Boase, Don A. Hunter, Kathy E. Schwinn
      Pages 63-74

  3. Biolistic DNA Delivery in Nematodes

    1. Front Matter

      Pages 75-75
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    2. Biolistic Transformation of Caenorhabditis elegans

      • Meltem Isik, Eugene Berezikov
      Pages 77-86

    3. Improved Vectors for Selection of Transgenic Caenorhabditis elegans

      • Annabel A. Ferguson, Liquan Cai, Luv Kashyap, Alfred L. Fisher
      Pages 87-102

    4. Biolistic Transformation of Brugia Malayi

      • Tarig B. Higazi, Thomas R. Unnasch
      Pages 103-115

  4. Biolistic DNA Delivery in Mammalian Cells In Vitro

    1. Front Matter

      Pages 117-117
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    2. Biolistic Transfection of Human Embryonic Kidney (HEK) 293 Cells

      • Xiongwei Li, Masaki Uchida, H. Oya Alpar, Peter Mertens
      Pages 119-132

    3. Biolistic Transfection of Tumor Tissue Samples

      • Kandan Aravindaram, Shu-Yi Yin, Ning-Sun Yang
      Pages 133-143

    4. Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes

      • David F. Steele, Ying Dou, David Fedida
      Pages 145-155

    5. Biolistic Transfection of Neurons in Organotypic Brain Slices

      • John A. O’Brien, Sarah C. R. Lummis
      Pages 157-166

  5. Biolistic DNA Delivery in Mammalian Cells In Vivo

    1. Front Matter

      Pages 167-167
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    2. Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun

      • Meng-Chi Yen, Ming-Derg Lai
      Pages 169-174

    3. Chemokine Overexpression in the Skin by Biolistic DNA Delivery

      • Ahmad Jalili
      Pages 175-188
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Keywords

About this book

Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity acceleration using a ballistic device such as the gene gun.  Biolistic DNA Delivery: Methods and Protocols provides a comprehensive collection of detailed protocols intended to provide the definitive practical guide for the novice as well as for the advanced gene transfer expert on how to introduce nucleic acids into eukaryotic cells using the biolistic technique. Split into six convenient sections, this detailed volume covers biolistic gene transfer into plants, nematodes, and mammalian cells, both in vitro and in vivo, as well as the use of gene gun-mediated DNA vaccination in various experimental animal models of human diseases, and the description of biolistic delivery of molecules other than nucleic acids. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

All-inclusive and cutting-edge,  Biolistic DNA Delivery: Methods and Protocols brings together the knowledge and the experience of leading experts in the field of gene transfer in order to serve all researchers who wish to further our abilities in this vital field.

Editors and Affiliations

  • Ganzimmun Diagnostics AG, Mainz, Germany

    Stephan Sudowe

  • University Medical Center, Department of Dermatology, Johannes Gutenberg-University, Mainz, Germany

    Angelika B. Reske-Kunz

Bibliographic Information

  • Book Title: Biolistic DNA Delivery

  • Book Subtitle: Methods and Protocols

  • Editors: Stephan Sudowe, Angelika B. Reske-Kunz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-110-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-109-7Published: 29 October 2012

  • Softcover ISBN: 978-1-4939-6068-2Published: 23 August 2016

  • eBook ISBN: 978-1-62703-110-3Published: 28 October 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 415

  • Topics: Human Genetics, Genetic Engineering

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