Abstract
Progressive motility is a vital functional characteristic of ejaculated human spermatozoa that governs their ability to penetrate into, and migrate through, both cervical mucus and the oocyte vestments, and ultimately fertilize the oocyte. A detailed protocol, based on the most common computer-aided sperm analysis (CASA) system with phase contrast microscope optics, is provided for performing reliable assessments of sperm movement pattern characteristics (“kinematics”) in semen. The protocol can also be used with washed sperm suspensions where, in addition, the percentages of motile and progressively motile spermatozoa can also be derived. Using CASA technology it is also possible to identify biologically, and hence clinically, important subpopulations of spermatozoa (e.g., those in semen with good mucus-penetrating characteristics, or those showing hyperactivation when incubated under capacitating conditions) by applying multi-parametric definitions on a cell-by-cell basis.
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References
Mortimer ST (1997) A critical review of the physiological importance and analysis of sperm movement in mammals. Hum Reprod Update 3:403–439
MacLeod J, Gold RZ (1951) The male factor in fertility and infertility. III. An analysis of motile activity in the spermatozoa of 1000 fertile men and 1000 men in infertile marriage. Fertil Steril 2:187–204
Mortimer D (1994) Practical laboratory andrology. Oxford University Press, New York, NY
Björndahl L, Mortimer D, Barratt CLR, Castilla JA, Menkveld R, Kvist U, Alvarez JG, Haugen TB (2010) A practical guide to basic laboratory andrology. Cambridge University Press, Cambridge
Aitken RJ, Sutton M, Warner P, Richardson DW (1985) Relationship between the movement characteristics of human spermatozoa and their ability to penetrate cervical mucus and zona-free hamster oocytes. J Reprod Fertil 73:441–449
Mortimer D, Pandya IJ, Sawers RS (1986) Relationship between human sperm motility characteristics and sperm penetration into human cervical mucus in vitro. J Reprod Fertil 78:93–102
Mortimer D (1990) Objective analysis of sperm motility and kinematics. In: Keel BA, Webster BW (eds) Handbook of the laboratory diagnosis and treatment of infertility. CRC, Boca Raton, FL, pp 97–133
David G, Serres C, Jouannet P (1981) Kinematics of human spermatozoa. Gamete Res 4:83–95
Katz DF, Davis RO (1987) Automatic analysis of human sperm motion. J Androl 8:170–181
Boyers SP, Davis RO, Katz DF (1989) Automated semen analysis. Curr Prob Obstet Gynecol Fertil XII 5:167–200
Davis RO, Katz DF (1993) Computer-aided sperm analysis: technology at a crossroads. Fertil Steril 59:953–955
Mortimer D, Aitken RJ, Mortimer ST, Pacey AA (1995) Workshop report: clinical CASA—the quest for consensus. Reprod Fertil Dev 7:951–959
ESHRE Andrology Special Interest Group (1996) Consensus workshop on advanced diagnostic andrology techniques. Hum Reprod 11:1463–1479
Mortimer D, Mortimer ST (1998) Value and reliability of CASA systems. In: Ombelet W, Bosmans E, Vandeput H, Vereecken A, Renier M, Hoomans EH (eds) Modern ART in the 2000s. Andrology in the nineties. Parthenon Publishing, Carnforth, pp 73–89
ESHRE Andrology Special Interest Group (1998) Guidelines on the application of CASA technology in the analysis of spermatozoa. Hum Reprod 13:142–145
Mortimer ST (2000) CASA-practical aspects. J Androl 21:515–524
Mortimer D, Mortimer ST (2005) Laboratory investigation of the infertile male. In: Brinsden PR (ed) A textbook of in-vitro fertilization and assisted reproduction, 3rd edn. Taylor & Francis, London, pp 61–91
Mortimer D (1986) Elaboration of a new culture medium for physiological studies on human sperm motility and capacitation. Hum Reprod 1:247–250
Chapeau C, Gagnon C (1987) Nitrocellulose and polyvinyl coatings prevent sperm adhesion to glass without affecting the motility of intact and demembranated human spermatozoa. J Androl 8:34–40
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Mortimer, D., Mortimer, S.T. (2013). Computer-Aided Sperm Analysis (CASA) of Sperm Motility and Hyperactivation. In: Carrell, D., Aston, K. (eds) Spermatogenesis. Methods in Molecular Biology, vol 927. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-038-0_8
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DOI: https://doi.org/10.1007/978-1-62703-038-0_8
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-037-3
Online ISBN: 978-1-62703-038-0
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