Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 927)
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Table of contents (45 protocols)
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Front Matter
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Semen Analysis Methods
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Front Matter
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Sperm DNA Damage Testing
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Front Matter
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Advanced Clinical Testing
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Front Matter
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Keywords
About this book
Deficiencies in sperm function are usually the result of spermatogenic defects. Spermatogenesis is a biologically complex and essential process during which spermatogonia undergo meiotic recombination, reduction of the genome to a haploid state, and extensive cellular modifications that result in a motile cell capable of traversing the female reproductive tract, withstanding various potential assaults to viability, and finally successfully fertilizing a mature oocyte to give rise to an embryo. Defects in any step of spermatogenesis or spermatogenesis can lead to male infertility, a disease that affects approximately 5-7% of the population. Spermiogenesis and Spermatogenesis: Methods and Protocols details protocols used in the study of spermatogenesis, clinical analytical protocols, and basic techniques used in clinical andrology laboratories, such as obtaining accurate results for a sperm count, and advanced procedures, such as genome-wide genetic study tools and evaluation of nuclear proteins. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
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Authoritative and easily accessible, Spermiogenesis and Spermatogenesis: Methods and Protocols is unique in its breadth, and will be a useful reference for clinicians and researchers alike.
Editors and Affiliations
Bibliographic Information
Book Title: Spermatogenesis
Book Subtitle: Methods and Protocols
Editors: Douglas T. Carrell, Kenneth I. Aston
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-038-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2013
Hardcover ISBN: 978-1-62703-037-3Published: 20 September 2012
Softcover ISBN: 978-1-4939-6267-9Published: 23 August 2016
eBook ISBN: 978-1-62703-038-0Published: 20 September 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVII, 554
Topics: Cell Biology, Embryology, Developmental Biology