Abstract
Antisperm antibodies (ASA) are one well-known cause of refractory infertility in both males and females. In females, a sperm immobilization test, which detects sperm-immobilizing antibodies indirectly in the patient’s serum, requires complement for the reaction and thus seems to be a more specific immunological reaction. In males, an immunobead test or a mixed antiglobulin reaction test, which detects ASA directly on the sperm surface, is a screening test because of the nonspecific reaction.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Shibahara H et al (2005) Diagnosis and treatment of immunologically infertile males with anti-sperm antibodies. Reprod Med Biol 4:133–141
Shibahara H et al (2009) Diagnosis and treatment of immunologically infertile women with sperm-immobilizing antibodies in their sera. J Reprod Immunol 83:139–144
Koyama K et al (1979) Effects of antisperm antibodies on sperm migration through cervical mucus. Excerpta Med Int Congr Series 512:705–708
Shibahara H et al (2007) Relationship between level of serum sperm immobilizing antibody and its inhibitory effect on sperm migration through cervical mucus in immunologically infertile women. Am J Reprod Immunol 57:142–146
Shibahara H et al (1995) Sperm immobilizing antibodies interfere with sperm migration from the uterine cavity through the fallopian tubes. Am J Reprod Immunol 34:120–124
Shibahara H et al (1991) Inhibition of sperm-zona pellucida tight binding by sperm immobilizing antibodies as assessed by the hemizona assay (HZA). Nihon Sanka Fujinka Gakkai Zasshi 43:237–238
Shibahara H et al (1993) Effects of sperm-immobilizing antibodies on sperm-zona pellucida tight binding. Fertil Steril 60:533–539
Shibahara H et al (1996) Diversity of the blocking effects of antisperm antibodies on fertilization in human and mouse. Hum Reprod 11:2595–2599
Shibahara H et al (2003) Diversity of the inhibitory effects on fertilization by anti-sperm antibodies bound to the surface of ejaculated human sperm. Hum Reprod 18:1469–1473
Taneichi A et al (2002) Sperm immobilizing antibodies in the sera of infertile women cause low fertilization rates and poor embryo quality in vitro. Am J Reprod Immunol 47:46–51
Taneichi A et al (2003) Effects of sera from infertile women with sperm immobilizing antibodies on fertilization and embryo development in vitro in mice. Am J Reprod Immunol 50:146–151
World Health Organization (ed) (2010) WHO laboratory manual for the examination and processing of human semen, 5th edn. WHO, Switzerland
Bronson R et al (1982) Detection of sperm specific antibodies on the spermatozoa surface by immunobead binding. Arch Androl 9:61
Isojima S et al (1968) Immunologic analysis of sperm immobilizing factor found in the sera of women with unexplained sterility. Am J Obstet Gynecol 101:677–683
Franklin RR, Dukes CD (1964) Antisperma-tozoal antibody and unexplained infertility. Am J Obstet Gynecol 89:6–9
Isojima S, Koyama K (1974) Quantitative estimation of sperm immobilizing antibody in the sera of women with sterility of unknown etiology: the 50% sperm immobilization unit (SI50). Excerpta Med Int Congr Series 370:10–15
Kobayashi S et al (1990) Correlation between quantitative antibody titers of sperm immobilizing antibodies and pregnancy rates by treatments. Fertil Steril 54:1107–1113
Isojima S, Koyama K (1989) Techniques for sperm immobilization test. Arch Androl 23:185–199
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Shibahara, H., Koriyama, J. (2013). Methods for Direct and Indirect Antisperm Antibody Testing. In: Carrell, D., Aston, K. (eds) Spermatogenesis. Methods in Molecular Biology, vol 927. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-038-0_6
Download citation
DOI: https://doi.org/10.1007/978-1-62703-038-0_6
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-037-3
Online ISBN: 978-1-62703-038-0
eBook Packages: Springer Protocols