Abstract
The growth is a characteristic of each culture and it is determinate by the origin of the species, culture conditions, and type of culture. In this chapter, we make a comparison of the different growth parameters among three different species and three different types of cultures.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Hall RD (1991) The initiation and maintenance of callus cultures of carrot and tobacco. In: Lindsey K (ed) Plant tissue culture manual. Supplement 3. Kluwer, The Netherlands, pp 1–19
Thorpe TA (1980) Organogenesis in vitro: structural, physiological and biochemical aspects. Int Rev Cytol Suppl 11A:71–111
Wagley LM, Gladfelter HJ, PHILLIPS GC (1987) De novo shoot organogenesis of Pinus eldarica Medw. in vitro. II. Macro- and micro-photographic evidence of de novo regeneration. Plant Cell Rep 6:167–171
Smith RH (1992) Plant tissue culture. Techniques and experiments. Academic, San Diego
Collin HA, Edwards S (1998) Plant cell culture. Springer, New York, NY
Hall RD (1991) The initiation and maintenance of plant cell suspension cultures. In: Lindsey K (ed) Plant tissue culture manual. Supplement 3. Kluwer, The Netherlands, pp 1–21
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158
Street HE (1977) Cell suspension cultures. Techniques. In: Street HE (ed) Plant tissue and cell culture. University of California Press, Oxford, pp 61–102
Morris P, Fowler MW (1981) A new method for the production of fine plant cell suspension cultures. Plant Cell Tiss Org Cult 1:15–24
Davis BD, Dulbecco R, Eisen HN et al (1978) Tratado de Microbiología. Con Inclusión de Inmunología y Genética Molecular. Salvat Editores, S.A., Barcelona
Phillips GC, Hubstenberger JF, Hansen EE (1995) Plant regeneration by organogenesis from callus and cell suspension cultures. In: Gamborg OL, Phillips GC (eds) Plant cell, tissue and organ culture. Fundamental methods. Springer-Verlag, Germany, pp 67–79
Coles GD, Abernethy DJ, Christey MC et al (1991) Monitoring hairy-root growth by image analysis. Plant Mol Biol Rep 9:13–20
Hahlbrock K, Kuhlen E (1972) Relationship between growth of parsley and soybean cells in suspension cultures and changes in the conductivity of the culture medium. Planta 108:271–278
James E, Lee JM (2000) An improved optical technique for monitoring plant cell concentration. Plant Cell Rep 19:283–285
Madhusudhan R, Rao SR, Ravishankar GA (1995) Osmolarity as a measure of growth of plant cells in suspension cultures. Enzyme Microb Technol 17:989–991
Mills DR, Lee JM (1996) A simple, accurate method for determining wet and dry weight concentrations of plant cell suspension cultures using microcentrifuge tubes. Plant Cell Rep 15:634–636
Nicoloso FT, Val J, Van der Keur M et al (1994) Flow-cytometric cell counting and DNA estimation for the study of plant cell population dynamics. Plant Cell Tiss Org Cult 39:251–259
Olofsdotter M (1993) Image processing: a non-destructive method for measuring growth in cell and tissue culture. Plant Cell Rep 12:216–219
Shetty K, Bothra D, Crawford DL et al (1990) Extracellular peroxidases as indicators of growth in plant cell suspension cultures. Appl Biochem Biotechnol 24(25):213–221
Sung ZR (1976) Turbidimetric measurement of plant cell culture growth. Plant Physiol 57:460–462
Vitecek J, Adam V, Petek J et al (2004) Esterases as a marker for growth of BY-2 tobacco cells and early somatic embryos of the Norway spruce. Plant Cell Tiss Org Cult 79:195–201
Huang C-N, Cornejo MJ, Bush DS et al (1986) Estimating viability of plant protoplasts using double and single staining. Protoplasma 135:80–87
Baker CJ, Mock NM (1994) An improved method for monitoring cell death in cell suspension and leaf disc assays using Evans blue. Plant Cell Tiss Org Cult 39:7–12
Widholm JM (1982) The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol 47:189–194
Suzuki T, Yoshioka T, Kato Y et al (1987) A new estimation method for plant cell viability by determining electron transport activity. Plant Cell Rep 6:279–282
Sowa S, Towill LE (1991) Infrared spectroscopy of plant cell cultures. Noninvasive measurement of viability. Plant Physiol 95:610–615
Steward N, Martin R, Engasser JM et al (1999) A new methodology for plant cell viability assessment using intracellular esterase activity. Plant Cell Rep 19:171–176
Escobedo-GraciaMedrano RM, Miranda-Ham ML (2003) Analysis of elicitor induced cell viability changes in Lycopersicon esculentum Mill suspension culture by different methods. In Vitro Cell Dev Biol-Plant 39:236–239
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Ciau-Uitz R, Miranda-Ham ML, Coello-Coello J et al (1994) Indole alkaloid production by transformed and non-transformed root cultures of Catharanthus roseus. In Vitro Cell Dev Biol-Plant 30:84–88
Acknowledgment
This work was supported by a grant from CONACYT for Jatropha work.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Galáz-Ávalos, R.M., Aguilar-Díaz, S., Xool-González, P.A., Huchín-May, S.M., Loyola-Vargas, V.M. (2012). Callus, Suspension Culture, and Hairy Roots. Induction, Maintenance and Characterization. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 877. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-818-4_3
Download citation
DOI: https://doi.org/10.1007/978-1-61779-818-4_3
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-817-7
Online ISBN: 978-1-61779-818-4
eBook Packages: Springer Protocols