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Image processing: a non-destructive method for measuring growth in cell and tissue culture

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Abstract

The use of machine vision in evaluating callus growth directly in Petri dishes is reported for carrot cell suspension growing in the presence of increasing concentrations of various herbicides. Machine vision was much more accurate and sensitive than visual counting of the cell aggregates. Image processing also gives more detailed information than does counting because of its ability to distinguish between intermediate levels of callus growth.

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Abbreviations

2,4-D:

2,4 dichlorophenoxyacetic acid

MS:

Murashige and Skoog

GR:

Gelrite

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Authors and Affiliations

  1. Department of Agricultural Sciences, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark

    Maria Olofsdotter

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  1. Maria Olofsdotter
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Communicated by W. Barz

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Olofsdotter, M. Image processing: a non-destructive method for measuring growth in cell and tissue culture. Plant Cell Reports 12, 216–219 (1993). https://doi.org/10.1007/BF00237057

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  • DOI: https://doi.org/10.1007/BF00237057

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