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Broad-Host-Range Plasmid Vectors for Gene Expression in Bacteria

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Strain Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 765))

Abstract

This chapter provides methods and insights into the use of broad-host-range plasmid vectors useful for expression of genes in a variety of bacteria. The main focus is on IncQ, IncW, IncP, and pBBR1-based plasmids which have all been used for such applications. The specific design of each vector is adapted to its use, and here we describe, as an example, a protocol for construction (in Escherichia coli) of large insert DNA libraries in an IncP type vector and transfer of the library to the desired host. The genes of interest will in this case have to be expressed from their own promoters and the libraries will be screened by a method that best fits the functions of the gene or gene clusters searched for.

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Author information

Authors and Affiliations

  1. Department of Biotechnology, Norwegian University of Science and Technology (NTNU), Trondheim, Norway

    Rahmi Lale & Svein Valla

  2. Department of Biotechnology, SINTEF Materials and Chemistry, Trondheim, Norway

    Trygve Brautaset

Authors
  1. Rahmi Lale
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  2. Trygve Brautaset
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  3. Svein Valla
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Correspondence to Svein Valla .

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Editors and Affiliations

  1. Nature Technology Corporation, Innovation Drive 4701, Lincoln, 68521, Nebraska, USA

    James A. Williams

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Lale, R., Brautaset, T., Valla, S. (2011). Broad-Host-Range Plasmid Vectors for Gene Expression in Bacteria. In: Williams, J. (eds) Strain Engineering. Methods in Molecular Biology, vol 765. Humana Press. https://doi.org/10.1007/978-1-61779-197-0_19

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  • DOI: https://doi.org/10.1007/978-1-61779-197-0_19

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-196-3

  • Online ISBN: 978-1-61779-197-0

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