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Methodologies to increase the transformation efficiencies and the range of bacteria that can be transformed

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Abstract

Methods for transfer of exogenous DNA into cells are essential for genetics and molecular biology, and the lack of effective methods hampers research on many different species of bacteria which have shown to be particularly recalcitrant to transformation. This review presents the progress on the development of methods for artificial transformation of bacteria with emphasis on different methodologies and the range of bacteria that can be transformed. The methods' strengths and weaknesses are described.

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Acknowledgments

FLA thanks the KMB project, Norwegian Research Council for financial support. Furthermore, we thank Svein Valla for proofreading the manuscript and for useful suggestions and comments.

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  1. Vectron Biosolutions AS, P.O. Box 1262, Pirsenteret, 7462, Trondheim, Norway

    Trond Erik Vee Aune

  2. Department of Biotechnology, NOBIPOL, Norwegian University of Science and Technology, Sem Sælands vei 6/8, 7491, Trondheim, Norway

    Finn Lillelund Aachmann

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Aune, T.E.V., Aachmann, F.L. Methodologies to increase the transformation efficiencies and the range of bacteria that can be transformed. Appl Microbiol Biotechnol 85, 1301–1313 (2010). https://doi.org/10.1007/s00253-009-2349-1

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