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Intracellular Calcium Assays in Dissociated Primary Cortical Neurons

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Protocols for Neural Cell Culture

Part of the book series: Springer Protocols Handbooks ((SPH))

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Abstract

In this chapter, the basic tools and methods to isolate neurons from the embryonic rat cortex are provided. We outline the isolation of fresh neuronal cells and their storage, post-thaw maintenance, and the application of techniques to measure intracellular calcium changes in response to the addition of neurotransmitters. Many of these techniques come in kit formats supplied by companies like Invitrogen and thus enhance the ease of use, time required, and reproducibility.

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Author information

Authors and Affiliations

  1. Invitrogen Corporation, Frederick, MD, USA

    Navjot Kaur

  2. Invitrogen Corporation, Madison, WI, USA

    David V. Thompson & David R. Piper

  3. Invitrogen Corporation, Grand Island, NY, USA

    David Judd & Richard G. Del Mastro

Authors
  1. Navjot Kaur
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  2. David V. Thompson
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  3. David Judd
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  4. David R. Piper
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  5. Richard G. Del Mastro
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Editor information

Editors and Affiliations

  1. Dept. Pathology & Molecular Medicine, McMaster University, Main Street West 1200, Hamilton, L8N 3Z5, Canada

    Laurie C. Doering

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Kaur, N., Thompson, D.V., Judd, D., Piper, D.R., Del Mastro, R.G. (2009). Intracellular Calcium Assays in Dissociated Primary Cortical Neurons. In: Doering, L. (eds) Protocols for Neural Cell Culture. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60761-292-6_7

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  • DOI: https://doi.org/10.1007/978-1-60761-292-6_7

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-291-9

  • Online ISBN: 978-1-60761-292-6

  • eBook Packages: Springer Protocols

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