Abstract
Complementary to other imaging techniques, MALDI MS imaging provides label-free analysis and chemical specificity, covering a wide range of molecules that can be visualized simultaneously. Here, we highlight a protocol to visualize lipids from single cardiac cells using high-resolution atmospheric-pressure matrix-assisted laser desorption/ionization coupled to an orbital trapping mass analyzer.
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Acknowledgments
Financial support by the Deutsche Forschungsgemeinschaft (DFG) under grant Sp314/13-1. M.F. and G.C. are financially supported by funding from the European Commission’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement number 675132 (MSCA-ITN-ETN MASSTRPLAN). Financial support from the German Federal Ministry of Education and Research (BMBF) within the framework of the e:Med research and funding concept for SysMedOS project (to MF) is gratefully acknowledged.
Competing financial interests: B.S. is a consultant to TransMIT GmbH Giessen. D.B. is a part-time employee of TransMIT GmbH.
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Bhandari, D.R., Coliva, G., Fedorova, M., Spengler, B. (2020). Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging. In: Shrestha, B. (eds) Single Cell Metabolism. Methods in Molecular Biology, vol 2064. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9831-9_8
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DOI: https://doi.org/10.1007/978-1-4939-9831-9_8
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