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Dental Pulp Stem Cells: Isolation, Characterization, Expansion, and Odontoblast Differentiation for Tissue Engineering

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Odontogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1922))

Abstract

Tissue engineering is an interdisciplinary area offering a promising approach by the use of stem cells combined with scaffolds and signaling factors for regeneration of damaged or lost tissues. Incorporation of a sufficient number of cells which do not elicit the immunoreaction in the body is a pivotal element for successful tissue formation using this method. Stem cells exhibiting strong capacity to self-renew and differentiate into different cell types are considered as a potent cell source. Among various cell sources, dental pulp stem cells (DPSCs) are widely under investigation due to the fact that they are simply obtainable from extracted third molars or orthodontically extracted teeth and show an excellent potential for clinical application and also their harvesting method is minimally invasive. DPSCs are odontogenic progenitor cells with clonogenic abilities, rapid proliferation rates, and multiple differentiation potentials. Here, we describe protocols that allow 1) the isolation of DPSCs from a single tooth; 2) the characterization of human mesenchymal stem cells markers of DPSCs by flow cytometry; 3) the culture growth of DPSCs in 2D (in cell culture flasks) and 3D (by 3D printing of cell-laden constructs); and 4) the in vivo evaluation of differentiation potential of DPSCs.

Qing Dong and Yuanyuan Wang contributed equally to this work

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Author information

Authors and Affiliations

  1. Department of Orthodontics and Pediatric Dentistry, School of Dentistry, University of Michigan, Ann Arbor, MI, USA

    Qing Dong, Yuanyuan Wang & Li Zheng

  2. College of Dentistry, University of Saskatchewan, Saskatoon, SK, Canada

    Fatemeh Mohabatpour & Petros Papagerakis

  3. Department of Mechanical Engineering, College of Engineering, University of Saskatchewan, Saskatoon, SK, Canada

    Fatemeh Mohabatpour & Daniel Chen

  4. Department of Surgery, College of Medicine, University of Saskatchewan, Saskatoon, SK, Canada

    Silvana Papagerakis

  5. Department of Otolaryngology Head and Neck Surgery, School of Medicine, University of Michigan, Ann Arbor, MI, USA

    Silvana Papagerakis

  6. Toxicology Interdisciplinary Program, University of Saskatchewan, Saskatoon, SK, Canada

    Silvana Papagerakis

  7. Biomedical Engineering, University of Saskatchewan, Saskatoon, SK, Canada

    Silvana Papagerakis

  8. Department of Pediatric Dentistry, College of Stomatology, North China University of Science and Technology, Tangshan, China

    Qing Dong

  9. School of Dentistry, University of Michigan, Ann Arbor, MI, USA

    Petros Papagerakis

Authors
  1. Qing Dong
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  2. Yuanyuan Wang
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  3. Fatemeh Mohabatpour
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  4. Li Zheng
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  5. Silvana Papagerakis
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  6. Daniel Chen
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  7. Petros Papagerakis
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Corresponding author

Correspondence to Petros Papagerakis .

Editor information

Editors and Affiliations

  1. School of Dentistry, University of Michigan, Ann Arbor, MI, USA

    Petros Papagerakis

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Dong, Q. et al. (2019). Dental Pulp Stem Cells: Isolation, Characterization, Expansion, and Odontoblast Differentiation for Tissue Engineering. In: Papagerakis, P. (eds) Odontogenesis. Methods in Molecular Biology, vol 1922. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9012-2_9

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  • DOI: https://doi.org/10.1007/978-1-4939-9012-2_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9011-5

  • Online ISBN: 978-1-4939-9012-2

  • eBook Packages: Springer Protocols

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