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Vacuole-Targeted Proteins: Ins and Outs of Subcellular Localization Studies

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Plant Vacuolar Trafficking

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1789))

Abstract

Accurate and efficient demonstrations of protein localizations to the vacuole or tonoplast remain strict prerequisites to decipher the role of vacuoles in the whole plant cell biology and notably in defence processes. In this chapter, we describe a reliable procedure of protein subcellular localization study through transient transformations of Catharanthus roseus or onion cells and expression of fusions with fluorescent proteins allowing minimizing artefacts of targeting.

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Acknowledgments

We gratefully acknowledge support from the University François-Rabelais of Tours and from the “Région Centre-Val de Loire” (France): ABISAL grant, CatharSIS program and BioPROPHARM Project – ARD2020 Biomédicaments). IC was financed by a postdoctoral fellowship from Région Centre Val de Loire. We thank Marie-Antoinette Marquet, Evelyne Danos, and Cédric Labarre (EA2106 BBV) for help in maintaining cell cultures and plants; Emeline Marais and Céline Mélin (BBV) for valuable technical assistance.

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Author notes

  1. Inês Carqueijeiro, Liuda J. Sepúlveda, and Angela Mosquera contributed equally to this work.

Authors and Affiliations

  1. EA2106 “Biomolécules et Biotechnologies Végétales”, UFR Sciences et Techniques, Université François-Rabelais de Tours, Tours, France

    Inês Carqueijeiro, Liuda J. Sepúlveda, Angela Mosquera, Cyrielle Corbin, Thomas Dugé de Bernonville, Sébastien Besseau, Arnaud Lanoue, Gaëlle Glévarec, Marc Clastre, Benoit St-Pierre, Nathalie Giglioli-Guivarc’h, Audrey Oudin & Vincent Courdavault

  2. Laboratorio de Biotecnología, Sede de Investigación Universitaria, Universidad de Antioquia, Medellin, Colombia

    Liuda J. Sepúlveda, Angela Mosquera & Lucia Atehortùa

  3. Department of Biological Chemistry, The John Innes Centre, Norwich Research Park, Norwich, UK

    Richard Payne & Sarah E. O’Connor

  4. EA3142 “Groupe d’Etude des Interactions Hôte-Pathogène”, Université d’Angers, Angers, France

    Nicolas Papon

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  1. Inês Carqueijeiro
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Corresponding author

Correspondence to Vincent Courdavault .

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  1. Faculty of Sciences, University of Porto, Porto, Portugal

    Cláudia Pereira

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Carqueijeiro, I. et al. (2018). Vacuole-Targeted Proteins: Ins and Outs of Subcellular Localization Studies. In: Pereira, C. (eds) Plant Vacuolar Trafficking. Methods in Molecular Biology, vol 1789. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7856-4_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7856-4_4

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