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Basophil Degranulation Assay

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Food Allergens

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1592))

Abstract

Basophil degranulation assay has gained importance over the last decade in both diagnosis of food allergy and evaluation of progression of immunotherapy. This assay involves the identification and quantification of the expression of CD63 molecule on basophil membrane. CD63 is a marker of multivesicular bodies that is exposed to cell membrane during the process of degranulation in which the contents of basophil granules are released. This chapter describes the methodology for performing this assay.

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Author information

Authors and Affiliations

  1. Division of Allergy and Immunology, Department of Pediatrics, The Jaffe Food Allergy Institute, Icahn School of Medicine at Mount Sinai, Icahn Building, Room 11-23B, Box 1089, New York, NY, 10029, USA

    Madhan Masilamani & Mohanapriya Kamalakannan

  2. Immunology Institute and The Mindich Child Health and Development Institute, Mount Sinai School of Medicine, New York, NY, 10029, USA

    Madhan Masilamani

  3. Pediatric Allergy and Immunology, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    Hugh A. Sampson

  4. The Jaffe Food Allergy Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    Hugh A. Sampson

Authors
  1. Madhan Masilamani
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  2. Mohanapriya Kamalakannan
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  3. Hugh A. Sampson
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Corresponding author

Correspondence to Madhan Masilamani .

Editor information

Editors and Affiliations

  1. Bioinformatics Institute, A*STAR, Singapore, Singapore

    Jing Lin

  2. School of Biosciences, University of Nottingham, Sutton Bonington Campus, Leicestershire, United Kingdom

    Marcos Alcocer

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Masilamani, M., Kamalakannan, M., Sampson, H.A. (2017). Basophil Degranulation Assay. In: Lin, J., Alcocer, M. (eds) Food Allergens. Methods in Molecular Biology, vol 1592. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6925-8_11

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  • DOI: https://doi.org/10.1007/978-1-4939-6925-8_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6923-4

  • Online ISBN: 978-1-4939-6925-8

  • eBook Packages: Springer Protocols

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