Book Volume 1592 2017

Food Allergens

Methods and Protocols

Editors:

ISBN: 978-1-4939-6923-4 (Print) 978-1-4939-6925-8 (Online)

Table of contents (20 protocols)

  1. Front Matter

    Pages i-x

  2. No Access

    Protocol

    Pages 1-9

    Overview of the Commonly Used Methods for Food Allergens

  3. Food Allergen Purification and Production

    1. Front Matter

      Pages 11-11

    2. No Access

      Protocol

      Pages 13-22

      Allergen Extraction and Purification from Natural Products: Main Chromatographic Techniques

    3. No Access

      Protocol

      Pages 23-45

      Recombinant Allergen Production in E. coli

    4. No Access

      Protocol

      Pages 47-56

      Recombinant Allergens Production in Yeast

  4. Food Allergen Discovery, Detection, and Quantification

    1. Front Matter

      Pages 57-57

    2. No Access

      Protocol

      Pages 59-69

      2D-Electrophoresis and Immunoblotting in Food Allergy

    3. No Access

      Protocol

      Pages 71-78

      Two-Dimensional Electrophoresis and Identification by Mass Spectrometry

    4. No Access

      Protocol

      Pages 79-94

      Enzyme-Linked Immunosorbent Assay (ELISA)

    5. No Access

      Protocol

      Pages 95-108

      Detection of Food Allergens by Taqman Real-Time PCR Methodology

    6. No Access

      Protocol

      Pages 109-128

      Detection of Food Allergens by Phage-Displayed Produced Antibodies

    7. No Access

      Protocol

      Pages 129-137

      Protein Microarray-Based IgE Immunoassay for Allergy Diagnosis

    8. No Access

      Protocol

      Pages 139-146

      Basophil Degranulation Assay

    9. No Access

      Protocol

      Pages 147-161

      Use of Humanized RS-ATL8 Reporter System for Detection of Allergen-Specific IgE Sensitization in Human Food Allergy

  5. Allergenic Epitope Mapping

    1. Front Matter

      Pages 163-163

    2. No Access

      Protocol

      Pages 165-175

      Assessment of IgE Reactivity of β-Casein by Western Blotting After Digestion with Simulated Gastric Fluid

    3. No Access

      Protocol

      Pages 177-187

      IgE Epitope Mapping Using Peptide Microarray Immunoassay

    4. No Access

      Protocol

      Pages 189-198

      T-Cell Proliferation Assay: Determination of Immunodominant T-Cell Epitopes of Food Allergens

    5. No Access

      Protocol

      Pages 199-209

      Tetramer-Guided Epitope Mapping: A Rapid Approach to Identify HLA-Restricted T-Cell Epitopes from Composite Allergens

    6. No Access

      Protocol

      Pages 211-222

      T-Cell Epitope Prediction

    7. No Access

      Protocol

      Pages 223-245

      An Overview of Bioinformatics Tools and Resources in Allergy

  6. Methods Currently Being Developed and Future Development

    1. Front Matter

      Pages 247-247

    2. No Access

      Protocol

      Pages 249-262

      The Use of a Semi-Automated System to Measure Mouse Natural Killer T (NKT) Cell Activation by Lipid-Loaded Dendritic Cells

    3. No Access

      Protocol

      Pages 263-295

      Recent Advances in the Detection of Allergens in Foods

  7. Back Matter

    Pages 297-299