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The Use of Cultured Drosophila Cells for Studying the Microtubule Cytoskeleton

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Mitosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1136))

Abstract

Cultured Drosophila cell lines have been developed into a powerful tool for studying a wide variety of cellular processes. Their ability to be easily and cheaply cultured as well as their susceptibility to protein knockdown via double-stranded RNA-mediated interference (RNAi) has made them the model system of choice for many researchers in the fields of cell biology and functional genomics. Here we describe basic techniques for gene knockdown, transgene expression, preparation for fluorescence microscopy, and centrosome enrichment using cultured Drosophila cells with an emphasis on studying the microtubule cytoskeleton.

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Authors and Affiliations

  1. Department of Cellular & Molecular Medicine, Arizona Cancer Center, Room 3951, University of Arizona, 1515 N. Campbell Avenue, Tucson, AZ, 85724, USA

    Jonathan Nye, Daniel W. Buster & Gregory C. Rogers

Authors
  1. Jonathan Nye
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  2. Daniel W. Buster
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  3. Gregory C. Rogers
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Corresponding author

Correspondence to Gregory C. Rogers .

Editor information

Editors and Affiliations

  1. Dept Physiology Biophysics, Albert Einstein College of Medicine, Bronx, New York, USA

    David J. Sharp

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Nye, J., Buster, D.W., Rogers, G.C. (2014). The Use of Cultured Drosophila Cells for Studying the Microtubule Cytoskeleton. In: Sharp, D. (eds) Mitosis. Methods in Molecular Biology, vol 1136. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0329-0_6

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  • DOI: https://doi.org/10.1007/978-1-4939-0329-0_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0328-3

  • Online ISBN: 978-1-4939-0329-0

  • eBook Packages: Springer Protocols

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