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Measuring Interferon Alpha and Other Cytokines in SLE

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Systemic Lupus Erythematosus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1134))

Abstract

The progression of disease in patients with systemic lupus erythematosus (SLE) is affected by production, accumulation, and actions of cytokines. Type I interferon (IFN), specifically IFN-α, is recognized as a central mediator of disease pathogenesis in SLE. We describe a functional assay to measure type I IFN activity in SLE plasma and have also measured the response of peripheral blood cells to that cytokine family. This method can be scaled to assess IFN functional activity, as well as activity and cellular response to other cytokines, in relation to cellular and serologic parameters relevant to SLE.

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Author information

Authors and Affiliations

  1. Division of Rheumatology, Hospital for Special Surgery, New York, NY, USA

    Mikhail Olferiev, Mari Lliguicota, Kyriakos A. Kirou & Mary K. Crow

Authors
  1. Mikhail Olferiev
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  2. Mari Lliguicota
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  3. Kyriakos A. Kirou
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  4. Mary K. Crow
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Editor information

Editors and Affiliations

  1. University of Exeter Medical School, Devon, United Kingdom

    Paul Eggleton

  2. Section of Immunology and Infection, Division of Applied Medicine, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom

    Frank J. Ward

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Olferiev, M., Lliguicota, M., Kirou, K.A., Crow, M.K. (2014). Measuring Interferon Alpha and Other Cytokines in SLE. In: Eggleton, P., Ward, F. (eds) Systemic Lupus Erythematosus. Methods in Molecular Biology, vol 1134. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0326-9_10

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  • DOI: https://doi.org/10.1007/978-1-4939-0326-9_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0325-2

  • Online ISBN: 978-1-4939-0326-9

  • eBook Packages: Springer Protocols

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