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Systemic Lupus Erythematosus

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Paul Eggleton

    1. University of Exeter Medical School, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  2. Frank J. Ward

    1. Section of Immunology and Infection, Division of Applied Medicine, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols in the MiMB standard format
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1134)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xvii
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  2. Pathology of Systemic Lupus Erythematosus: The Challenges Ahead

    • Pedro Correia Azevedo, Grainne Murphy, David A. Isenberg
    Pages 1-16

  3. The Lupus Biomarker Odyssey: One Experience

    • Joseph M. Ahearn, Susan Manzi, Chau-Ching Liu
    Pages 17-35

  4. Detection of Antinuclear Antibodies in SLE

    • Yashwant Kumar, Alka Bhatia
    Pages 37-45

  5. Tests for Circulating Immune Complexes

    • Mark H. Wener
    Pages 47-57

  6. Methods for Measuring Antibody-Dependent Cell-Mediated Cytotoxicity In Vitro

    • Aaron S. Miller, Max L. Tejada, Hélène Gazzano-Santoro
    Pages 59-65

  7. Recombinant Antibody Microarray for Profiling the Serum Proteome of SLE

    • Carl A. K. Borrebaeck, Gunnar Sturfelt, Christer Wingren
    Pages 67-78

  8. Bifunctional Antibody Fragment-Based Fusion Proteins for the Targeted Elimination of Pathogenic T-Cell Subsets

    • Wijnand Helfrich, Edwin Bremer
    Pages 79-93

  9. TLC Immunostaining for Detection of “Antiphospholipid” Antibodies

    • Fabrizio Conti, Cristiano Alessandri, Francesca Romana Spinelli, Antonella Capozzi, Francesco Martinelli, Serena Recalchi et al.
    Pages 95-101

  10. Induced Murine Models of Systemic Lupus Erythematosus

    • Yuan Xu, Leilani Zeumer, Westley H. Reeves, Laurence Morel
    Pages 103-130

  11. Measuring Interferon Alpha and Other Cytokines in SLE

    • Mikhail Olferiev, Mari Lliguicota, Kyriakos A. Kirou, Mary K. Crow
    Pages 131-150

  12. Detection of SLE Antigens in Neutrophil Extracellular Traps (NETs)

    • Carmelo Carmona-Rivera, Mariana J. Kaplan
    Pages 151-161

  13. Detection and Characterization of Autoantibodies Against Modified Self-Proteins in SLE Sera After Exposure to Reactive Oxygen and Nitrogen Species

    • Brent J. Ryan, Paul Eggleton
    Pages 163-171

  14. Generation of Self-Peptides to Treat Systemic Lupus Erythematosus

    • Jean-Paul Briand, Nicolas Schall, Sylviane Muller
    Pages 173-192

  15. Measurement of Malondialdehyde, Glutathione, and Glutathione Peroxidase in SLE Patients

    • Tamer A. Gheita, Sanaa A. Kenawy
    Pages 193-199

  16. Evaluating a Particular Circulating MicroRNA Species from an SLE Patient Using Stem-Loop qRT-PCR

    • Weiguo Sui, Fuhua Liu, Jiejing Chen, Minglin Ou, Yong Dai
    Pages 201-209

  17. Microarray Technology for Analysis of MicroRNA Expression in Renal Biopsies of Lupus Nephritis Patients

    • Weiguo Sui, Fuhua Liu, Jiejing Chen, Minglin Ou, Yong Dai
    Pages 211-220

  18. Laboratory Tests for the Antiphospholipid Syndrome

    • Charis Pericleous, Vera M. Ripoll, Ian Giles, Yiannis Ioannou
    Pages 221-235

  19. Isolation, Polarization, and Expansion of CD4+ Helper T Cell Lines and Clones Using Magnetic Beads

    • Lekh N. Dahal, Robert N. Barker, Frank J. Ward
    Pages 237-247

  20. Meta-analysis as a Diagnostic Tool for Predicting Disease Onset and/or Activity in Systemic Lupus Erythematosus

    • Isabel Cottrell, Asma Khan, Sidra Maqsood, Jemma Thornes, Paul Eggleton
    Pages 249-259
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Keywords

About this book

Systemic Lupus Erythematosus: Methods and Protocols describe a number of genetic, biochemical and immunological techniques. These techniques provide an advancing understanding of the pathology, breakdown of the immune system and therapeutic challenges of SLE in both humans and animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Systemic Lupus Erythematosus: Methods and Protocols appeal to biomedical and clinical scientists in a number of pathology disciplines at the doctoral and post-doctoral level.

Editors and Affiliations

  • University of Exeter Medical School, United Kingdom

    Paul Eggleton

  • Section of Immunology and Infection, Division of Applied Medicine, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom

    Frank J. Ward

Bibliographic Information

  • Book Title: Systemic Lupus Erythematosus

  • Book Subtitle: Methods and Protocols

  • Editors: Paul Eggleton, Frank J. Ward

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0326-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0325-2Published: 05 February 2014

  • Softcover ISBN: 978-1-4939-5262-5Published: 23 August 2016

  • eBook ISBN: 978-1-4939-0326-9Published: 04 February 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 263

  • Number of Illustrations: 13 b/w illustrations, 22 illustrations in colour

  • Topics: Immunology, Pathology

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