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Cultivation of Adipose-Derived Stromal Cells on Intact Amniotic Membrane-Based Scaffold for Skin Tissue Engineering

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Skin Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1879))

Abstract

Application of cell-based skin substitutes has recently evolved as a novel treatment for hard-to-heal wounds. Here, we focus on the development of a novel skin substitute by seeding human adipose-derived stromal cells (ASCs) on acellular human amniotic membrane (HAM). This construction is probably associated with higher rates of host cell infiltration and implanted cell engraftment. ASCs are achieved by separation of stromal cells from lipoaspirates using collagenase digestion and acellular HAM was obtained by separation of outer membrane of the chorion and removing its epithelial cells.

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Acknowledgments

The protocols described here were developed with the support of Royan Institute grant.

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Authors and Affiliations

  1. Department of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

    Ehsan Taghiabadi, Nasser Aghdami & Amir Bajouri

  2. Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Ehsan Taghiabadi & Bahareh Beiki

Authors
  1. Ehsan Taghiabadi
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  2. Bahareh Beiki
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  3. Nasser Aghdami
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  4. Amir Bajouri
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Corresponding author

Correspondence to Amir Bajouri .

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Editors and Affiliations

  1. Ottawa, ON, Canada

    Kursad Turksen

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© 2018 Springer Science+Business Media New York

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Taghiabadi, E., Beiki, B., Aghdami, N., Bajouri, A. (2018). Cultivation of Adipose-Derived Stromal Cells on Intact Amniotic Membrane-Based Scaffold for Skin Tissue Engineering. In: Turksen, K. (eds) Skin Stem Cells. Methods in Molecular Biology, vol 1879. Humana Press, New York, NY. https://doi.org/10.1007/7651_2018_173

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  • DOI: https://doi.org/10.1007/7651_2018_173

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8869-3

  • Online ISBN: 978-1-4939-8870-9

  • eBook Packages: Springer Protocols

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