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Quantitative Efferocytosis Assays

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Phagocytosis and Phagosomes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1519))

Abstract

Efferocytosis, the phagocytic removal of apoptotic cells, is a dynamic process requiring recruitment of numerous regulatory proteins to forming efferosomes in a tightly regulated manner. Herein we describe microscopy-based methods for the enumeration of efferocytic events and characterization of the spatiotemporal dynamics of signaling molecule recruitment to efferosomes, using genetically encoded probes and immunofluorescent labeling. While these methods are illustrated using macrophages, they are applicable to any efferocytic cell type.

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Authors and Affiliations

  1. Department of Microbiology and Immunology and the Centre for Human Immunology, The University of Western Ontario, London, ON, N6A 5C1, Canada

    Amanda L. Evans, Jack W. D. Blackburn, Charles Yin & Bryan Heit

Authors
  1. Amanda L. Evans
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  2. Jack W. D. Blackburn
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  3. Charles Yin
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  4. Bryan Heit
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Corresponding author

Correspondence to Bryan Heit .

Editor information

Editors and Affiliations

  1. Department of Chemistry and Biology, Ryerson University, Toronto, Ontario, Canada

    Roberto Botelho

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Evans, A.L., Blackburn, J.W.D., Yin, C., Heit, B. (2017). Quantitative Efferocytosis Assays. In: Botelho, R. (eds) Phagocytosis and Phagosomes. Methods in Molecular Biology, vol 1519. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6581-6_3

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  • DOI: https://doi.org/10.1007/978-1-4939-6581-6_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6579-3

  • Online ISBN: 978-1-4939-6581-6

  • eBook Packages: Springer Protocols

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