Abstract
Connexins are the structural proteins of gap junctions and their functioning as tumor suppressors is well known. Epigenetic modifications, such as methylation of connexin genes, play important roles in regulating gene expression. Over the past decade, several methods have been applied to characterize DNA methylation-specific loci of connexin genes. This chapter describes analysis of selective connexin32 and connexin43 gene DNA methylation in human gastric tissues using methylation-specific PCR, bisulfite-specific PCR sequencing as well as MassArray techniques.
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Acknowledgements
The authors are grateful to the Endoscopic Unit of Third Xiangya Hospital of Central South University for the supply of clinical samples. This work was financially supported by the National Natural Science Foundation of China (No. 81172301).
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Liu, X., Xu, C. (2016). DNA Methylation Analysis of Human Tissue-Specific Connexin Genes. In: Vinken, M., Johnstone, S. (eds) Gap Junction Protocols. Methods in Molecular Biology, vol 1437. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3664-9_2
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DOI: https://doi.org/10.1007/978-1-4939-3664-9_2
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