Abstract
Analysing cell migration and invasion is of interest to many investigators as they mimic a part of physiological or pathological events. In this chapter, methods to analyse MMP-dependent 2D and 3D cell migration are described in detail. To study 2D cell migration, the phagokinetic track motility assay can be used. To study 3D cell migration, Matrigel invasion assay or microcarrier beads invasion assay can be used to obtain quantitative results.
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Acknowledgments
This work is supported by Cancer research UK project grant C1507/A2670. We thank Rob Visse for critical reading of the manuscript.
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Palmisano, R., Itoh, Y. (2010). Analysis of MMP-Dependent Cell Migration and Invasion. In: Clark, I. (eds) Matrix Metalloproteinase Protocols. Methods in Molecular Biology, vol 622. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-299-5_23
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DOI: https://doi.org/10.1007/978-1-60327-299-5_23
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