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Biotechnologies for Plant Mutation Breeding

Protocols

  • Book
  • Open Access
  • © 2017

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Overview

  • Open Access provided with a grant from the International Atomic Energy Agency
  • All contributions have been peer-reviewed
  • An excellent reference source for plant breeders, geneticists and seed companies as well as for undergraduate and postgraduate students specializing in agriculture, biotechnology, and molecular breeding

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Table of contents (20 chapters)

  1. Introduction

  2. Mutation Induction and Chimera Dissociation

  3. Phenotypic Screening

  4. Genotypic Screening

Keywords

About this book

This book is open access under a CC BY-NC 2.5 license.

This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.

Editors and Affiliations

  • Plant Breeding and Genetics Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna, Austria

    Joanna Jankowicz-Cieslak, Bradley J. Till

  • Crops Pathology and Genetics Research Unit, USDA-ARS, Davis, USA

    Thomas H. Tai

  • Plant Reproductive Biology, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany

    Jochen Kumlehn

Bibliographic Information

  • Book Title: Biotechnologies for Plant Mutation Breeding

  • Book Subtitle: Protocols

  • Editors: Joanna Jankowicz-Cieslak, Thomas H. Tai, Jochen Kumlehn, Bradley J. Till

  • DOI: https://doi.org/10.1007/978-3-319-45021-6

  • Publisher: Springer Cham

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: International Atomic Energy Agency 2017

  • Hardcover ISBN: 978-3-319-45019-3Published: 16 December 2016

  • Softcover ISBN: 978-3-319-83188-6Published: 04 July 2018

  • eBook ISBN: 978-3-319-45021-6Published: 08 December 2016

  • Edition Number: 1

  • Number of Pages: XX, 340

  • Number of Illustrations: 7 b/w illustrations, 69 illustrations in colour

  • Topics: Plant Breeding/Biotechnology, Agriculture, Genetic Engineering, Plant Genetics and Genomics

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