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Distribution and Expression Pattern of Claudins 6, 7, and 9 in Diffuse- and Intestinal-Type Gastric Adenocarcinomas

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Abstract

Introduction

Intestinal- and diffuse-type gastric adenocarcinomas differ in clinical outcome and genetic profile. Abnormal claudin expression has been well documented in several malignancies. Our aim was to find specific claudin markers for each type.

Methods

Fifty paraffin-embedded tissue blocks of diffuse- and intestinal-type gastric adenocarcinomas and fresh gastric biopsies obtained endoscopically from 20 patients with a presumptive diagnosis of gastric cancer were analyzed. Claudin-specific polyclonal and monoclonal antibodies were used for immunohistochemistry and Western blot analysis in total lysate and subcellular fractions.

Results

Claudin-6 expression was high in both types. Claudin-7 was expressed mainly in the diffuse-type whereas claudin-9 was mainly found in the apical membrane of the gland cells in the intestinal-type. Strong claudin-9 expression was associated with higher mortality rate (66%) in the diffuse type vs the intestinal type (25%) after a 2-year follow-up.

Conclusion

Claudins 6, 7, and 9 expressions are closely related to gastric carcinogenesis, and their detection is a useful prognostic marker in “intestinal-” and “diffuse-type” gastric adenocarcinomas.

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Acknowledgements

The authors are truly grateful to Dr. L. Cisneros and PS Miguel-Perez, Pathology Service HR “Lic. Adolfo López Mateos”, ISSSTE for collecting the gastric biopsies from the archives and to Veronica Rodriguez-Mata from the Department of Cellular and Tissular Biology, School of Medicine, UNAM for the histology processing of the samples.

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Authors and Affiliations

  1. Depto Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, México, DF, México

    Erika Rendón-Huerta, Alvarez-Fernández Georgina & Zavala-Zendejas Veronica

  2. Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México, México, DF, México

    Fortoul Teresa & Luis Felipe Montaño

  3. Depto Patología, Centro Médico Nacional, “20 de Noviembre”, ISSSTE, México, DF, México

    Gorráez María Teresa

  4. Servicio Gastroenterología, HR “Lic. Adolfo López Mateos”, ISSSTE, México, DF, México

    Garcia-Samper Xochitl

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  1. Erika Rendón-Huerta
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  2. Fortoul Teresa
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Correspondence to Luis Felipe Montaño.

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Financial support

This work was supported by grants IN-207108 and IN-202508/3 from DGAPA-UNAM and CONACYT-Salud-2006-13970, México.

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Rendón-Huerta, E., Teresa, F., Teresa, G.M. et al. Distribution and Expression Pattern of Claudins 6, 7, and 9 in Diffuse- and Intestinal-Type Gastric Adenocarcinomas. J Gastrointest Canc 41, 52–59 (2010). https://doi.org/10.1007/s12029-009-9110-y

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  • DOI: https://doi.org/10.1007/s12029-009-9110-y

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