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Regulation of the Expression of the Prostate-specific Antigen by Claudin-7

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Abstract

Claudins are a family of proteins involved in forming tight junctions between cells. Here we describe two forms of claudin-7 (CLDN-7), a full-length form of CLDN-7 with 211 amino-acid residues and a C-terminal truncated form with 158 amino-acid residues. These two forms of CLDN-7 are able to regulate the expression of a tissue-specific protein, the prostate-specific antigen (PSA), in the LNCaP prostate cancer cell line. We also found that the expression of CLDN-7 is responsive to androgen stimulation in the LNCaP cell line, suggesting that this protein is involved in the regulatory mechanism of androgen. Both forms of claudin-7 are expressed in human prostate, kidney and lung samples, and in most samples, the full-length form of claudin-7 was predominant. However, in some prostate samples from healthy individuals, the truncated form of claudin-7 is predominantly expressed. Our results demonstrated that unlike other claudins, CLDN-7 has both structural and regulatory functions, and the two forms of CLDN-7 may be related to cell differentiation in organ development.

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Authors and Affiliations

  1. UCLA Dental Research Institute and UCLA School of Dentistry, 10833 Le Conte Avenue, Los Angeles, CA 90095-1668, USA

    J. -Y. Zheng, D. Yu, M. Foroohar, E. Ko, J. Chan, N. Kim, R. Chiu & S. Pang

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  1. J. -Y. Zheng
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  2. D. Yu
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  3. M. Foroohar
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Correspondence to S. Pang.

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Zheng, JY., Yu, D., Foroohar, M. et al. Regulation of the Expression of the Prostate-specific Antigen by Claudin-7 . J. Membrane Biol. 194, 187–197 (2003). https://doi.org/10.1007/s00232-003-2038-4

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  • DOI: https://doi.org/10.1007/s00232-003-2038-4

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