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Silver-particle-based surface-enhanced resonance Raman scattering spectroscopy for biomolecular sensing and recognition

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Abstract

We demonstrate in this work that 2-μm-sized Ag (μAg) powders can be used as a core material for constructing biomolecular sensing/recognition units operating via surface-enhanced resonance Raman scattering (SERRS). This is possible because μAg powders are very efficient substrates for both the diffuse reflectance IR and the surface-enhanced Raman scattering–SERRS spectroscopic characterization of molecular adsorbates prepared in a similar manner on silver surfaces. Besides, the agglomeration of μAg particles in a buffer solution can be prevented by the layer-by-layer deposition of cationic and anionic polyelectrolytes such as poly(allylamine hydrochloride) (PAH) and poly(acrylic acid) (PAA). In this particular study, we used rhodamine B isothiocyanate (RhBITC) as a SERRS marker molecule, and μAg powders adsorbed consecutively with RhBITC and PAH–PAA bilayers were finally derivatized with biotinylated poly(l-lysine). On the basis of the nature of the SERRS peaks of RhBITC, those μAg powders were confirmed to selectively recognize streptavidin molecules down to concentrations of 10−10 g mL−1. Since a number of different molecules can be used as SERS–SERRS marker molecules, the present method proves to be an invaluable tool for multiplex biomolecular sensing/recognition via SERS and SERRS.

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Acknowledgement

This work was supported by the Korea Science and Engineering Foundation (grant no. R01-2006-000-10017-0).

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Authors and Affiliations

  1. Laboratory of Intelligent Interfaces, Department of Chemistry, Seoul National University, Seoul, 151-742, South Korea

    Kwan Kim, Hyun Sook Lee & Nam Hoon Kim

Authors
  1. Kwan Kim
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  2. Hyun Sook Lee
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  3. Nam Hoon Kim
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Correspondence to Kwan Kim.

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Kim, K., Lee, H.S. & Kim, N.H. Silver-particle-based surface-enhanced resonance Raman scattering spectroscopy for biomolecular sensing and recognition. Anal Bioanal Chem 388, 81–88 (2007). https://doi.org/10.1007/s00216-007-1182-6

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  • DOI: https://doi.org/10.1007/s00216-007-1182-6

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