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Genetic similarity and differences between the Orobanche cumana Wallr. Populations from Russia, Kazakhstan, and Romania revealed using the markers of simple sequence repeat

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Abstract

The molecular genetic diversity of the broomrape (O. cumana) populations, parasitizing on sunflower in Russia, Romania and Kazakhstan, was studied with codominant microsatellite markers. By means of cluster analysis, the populations of broomrape were divided into two clusters, regardless of their racial composition. In the first cluster, 19 samples from Russia and Kazakhstan, representing one gene pool are grouped; the second cluster consisted of five populations from Romania, united in another pool. According to Nei, the genetic distance between the clusters was 0.137. The analysis of molecular variance revealed that 22% of total variance were caused by differences between the genetic pools and 78% appeared due to differences between the samples within the each gene pool. The pairwise comparisons performed by Wright’s statistics showed that the differences between these two gene pools are sufficient (Fst = 21.9%) in order to make the conclusion about the existence of small genetic differentiation between the pools. The descriptive population genetic statistics for each pool indicate that the broomrape populations from Russia and Kazakhstan are characterized by a higher level of intrapopulation diversity than the populations from Romania.

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References

  1. Antonova, T.S., Araslanova, N.M., Strelnikov, E.A., Ramazanova, S.A., Guchetl, S.Z., and Chelyustnikova, T.A., Distribution of highly virulent races of sunflower broomrape (Orobanche Cumana Wallr.) in the southern regions of the Russian Federation, Russ. Agric. Sci., 2013, vol. 39, no. 1, pp. 46–50.

    Article  Google Scholar 

  2. Fernandez-Martinez, J.M., Velasco, L., and Perez-Vich, B., Progress in research on breeding for resistance to broomrape, in Proc. 18th Int. Sunflower Conf., Mar Del Plata & Balcarce, Argentina, Paris, France: Int. Sunfl. Assoc., 2012, pp. 57–62.

    Google Scholar 

  3. Škorić, D., Pǎcureanu-Joiţa, M., and Sava, E., Sunflower breeding for resistance to broomrape (Orobanche cumana Wallr.), An. Inst. Cercet. Cereale Plante Teh.-Fundulea, Acad. Stiinte Agric. Silvice, 2010, vol. 78, no. 1, pp. 63–79.

    Google Scholar 

  4. Ciuca, M., Pacureanu, M., and Iuora, M., RAPD markers for polymorphism identification in parasitic weed Orobanche cumana Wallr., Rom. Agric. Res., 2004, vol. 21, pp. 29–32.

    Google Scholar 

  5. Atanasova, R., Batcharova, R., Todorovska, E., and Atanassov, A., Molecular study of broomrape (Orobanche spp.) by RAPD analyses, Biotechnol. Biotechnol. Equip., 2005, vol. 19, no. 3, pp. 51–60.

    Article  CAS  Google Scholar 

  6. Benharrat, H., Veronesi, C., Theodet, C., and Thalouarn, P., Orobanche species and population discrimination using intersimple sequence repeat (ISSR), Weed Res., 2002, vol. 42, nos. 1–6, pp. 470–475.

    Article  CAS  Google Scholar 

  7. Gagne, G., Roeckel-Drevet, P., Grezes-Besset, B., Shindrova, P., Ivanov, P., Grand-Ravel, C., Vear, F., Tourvieille de Labrouhe, D., Charmet, G., and Nicolas, P., Study of the variability and evolution of Orobanche cumana populations infesting sunflower in different European countries, Theor. Appl. Genet., 1998, vol. 96, pp. 1216–1222.

    Article  Google Scholar 

  8. Pineda-Martos, R., Velasco, L., Fernandez-Escobar, J., Fernandez-Martinez, J.M., and Perez-Wich, B., Genetic diversity of Orobanche cumana populations from Spain assessed using SSR markers, Weed Res., 2013, vol. 43, pp. 279–289.

    Article  Google Scholar 

  9. Molinero-Ruiz, L., Garcia-Carneros, A.B., ColladoRomero, M., Rarancius, S., Dominguez, J., and Melero-Vara, J.M., Pathogenic and molecular diversity in highly virulent populations of the parasitic weed Orobanche cumana (sunflower broomrape) from Europe, Weed Res., 2014, vol. 55, pp. 87–96.

    Article  Google Scholar 

  10. Doyle, J.J. and Doyle, J.L., A rapid DNA isolation procedure for small quantities of fresh leaf tissue, Phytochem. Bull., 1987, vol. 19, pp. 11–15.

    Google Scholar 

  11. Guchetl, S.Z., Tchelustnikova, T.A., Araslanova, N.M., and Antonova, T.S., SSR and SCAR gene mapping of collection of national samples of sunflowers resistant and sensitive to race E Orobanche cumana Wallr., Sci. Techn. Bull. Russ. Sci. Res. Inst. Oil Cult., Oil Crops, 2012, no. 1 (150). pp. 20–26.

    Google Scholar 

  12. Peakall, R. and Smouse, P.E., GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update, Bioinfomatics, 2012, pp. 1–3.

    Google Scholar 

  13. Vaz Patto, M.C., Diaz-Ruiz, R., Satovic, Z., Roman, B., Pujadas-Salva, A.U., and Rubiales, D., Genetic diversity of Moroccan populations of Orobanche foetida: evolving from parasitizing wild hosts to crop plants, Weed Res., 2008, vol. 48, pp. 179–186.

    Article  Google Scholar 

  14. Roman, B., Perez-de-Luque, A., Alfaro, C., et al., Host differentiation in Orobanche foetida Poir, Flora — Morphol., Distrib. Funct. Ecol. Plants, 2007, vol. 202, pp. 201–208.

    Article  Google Scholar 

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Authors and Affiliations

  1. Pustovoit All-Russia Scientific Research Institute of Oil-bearing Crops, ul. Filatova 17, Krasnodar, 350038, Russia

    S. Z. Guchetl, T. S. Antonova & T. A. Tchelustnikova

Authors
  1. S. Z. Guchetl
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  2. T. S. Antonova
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  3. T. A. Tchelustnikova
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Correspondence to S. Z. Guchetl.

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Original Russian Text © S.Z. Guchetl, T.S. Antonova, T.A. Tchelustnikova, 2014, published in Doklady Rossiiskoi Akademii Sel’skokhozyaistvennykh Nauk, 2014, No. 4, pp. 23–27.

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Guchetl, S.Z., Antonova, T.S. & Tchelustnikova, T.A. Genetic similarity and differences between the Orobanche cumana Wallr. Populations from Russia, Kazakhstan, and Romania revealed using the markers of simple sequence repeat. Russ. Agricult. Sci. 40, 326–330 (2014). https://doi.org/10.3103/S1068367414050103

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