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Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29

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Abstract

An antifungal protein was isolated from a culture of Bacillus subtilis strain B29. The isolation procedure comprised ion exchange chromatography on diethylaminoethyl (DEAE)-52 cellulose and gel filtration chromatography on Bio-Gel® P-100. The protein was absorbed on DEAE-cellulose and Bio-Gel® P-100. The purified antifungal fraction was designated as B29I, with a molecular mass of 42.3 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), pI value 5.69 by isoelectric focusing (IEF)-PAGE, and 97.81% purity by high performance liquid chromatography (HPLC). B29I exhibited inhibitory activity on mycelial growth in Fusarium oxysporum, Rhizoctonia solani, Fusarium moniliforme, and Sclerotinia sclerotiorum. The 50% inhibitory concentrations (IC50) of its antifungal activity toward Fusarium oxysporum and Rhizoctonia solani were 45 and 112 μmol/L, respectively. B29I also demonstrated an inhibitory effect on conidial spore germination of Fusarium oxysporum and suppression of germ-tube elongation, and induced distortion, tumescence, and rupture of a portion of the germinated spores.

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Authors and Affiliations

  1. Department of Life Science and Engineering, Harbin Institute of Technology, Harbin, 150001, China

    Jing Li & Qian Yang

  2. Institute of Microbiology of Heilongjiang Academy of Sciences, Harbin, 150010, China

    Jing Li, Shu-mei Zhang, Yu-xia Wang & Xiao-yu Zhao

  3. Heilongjiang University Press, Harbin, 150040, China

    Li-hua Zhao

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  1. Jing Li
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  2. Qian Yang
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  3. Li-hua Zhao
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  4. Shu-mei Zhang
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  5. Yu-xia Wang
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  6. Xiao-yu Zhao
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Correspondence to Qian Yang.

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Project supported by the Hi-Tech Research and Development Program (863) of China (No. 2003AA241140) and the Natural Science Foundation of Heilongjiang Province, China (No. C200522)

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Li, J., Yang, Q., Zhao, Lh. et al. Purification and characterization of a novel antifungal protein from Bacillus subtilis strain B29. J. Zhejiang Univ. Sci. B 10, 264–272 (2009). https://doi.org/10.1631/jzus.B0820341

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  • DOI: https://doi.org/10.1631/jzus.B0820341

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