Abstract
Response surface methodology (RSM) was used to optimize the fermentation medium for enhancing pyruvic acid production by Torulopsis glabrata TP19. In the first step of optimization, with Plackett-Burman design, ammonium sulfate, glucose and nicotinic acid were found to be the important factors affecting pyruvic acid production significantly. In the second step, a 23 full factorial central composite design and RSM were applied to determine the optimal concentration of each significant variable. A second-order polynomial was determined by the multiple regression analysis of the experimental data. The optimum values for the critical components were obtained as follows: ammonium sulfate 0.7498 (10.75 g/L), glucose 0.9383 (109.38 g/L) and nicotinic acid 0.3633 (7.86 mg/L) with a predicted value of maximum pyruvic acid production of 42.2 g/L. Under the optimal conditions, the practical pyruvic acid production was 42.4 g/L. The determination coefficient (R 2) was 0.9483, which ensures adequate credibility of the model. By scaling up fermentation from flask to jar fermentor, we obtained promising results.
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References
Adinarayana, K., Ellaiah, P., 2002. Response surface optimization of the critical medium components for this production of alkaline protease by a newly isolated Bacillus sp. J. Pharm. Pharm. Sci., 5(3):272–227.
Ai, M., Ohdan, K., 1995. Formation of pyruvic acid by oxidative dehydrogenation of lactic acid. Chem. Lett., 24(5):405. [doi:10.1246/cl.1995.405]
Burdick, B.A., Schaeffer, J.R., 1987. Co-immobilized coupled enzyme systems on nylon mesh capable of gluconic and pyruvic acid production. Biotechnol. Lett., 9(4):253–258. [doi:10.1007/BF01027159]
Causey, T.B., Shanmugam, K.T., Yomano, L.P., Inram, L.O., 2004. Engineering Escherichia coli for efficient conversion of glucose to pyruvate. Proc. Natl. Acad. Sci. USA, 101(8):2235–2240. [doi:10.1073/pnas.0308171100]
Chakravarti, R., Sahai, V., 2002. Optimization of compactin production in chemically defined production medium by Penicillium citrinum using statistical methods. Process Biochem., 38(4):481–486. [doi:10.1016/S0032-9592(02)00138-3]
Conley, W.C., 1984. Computer Optimization Techniques. Petrocelli Books, Princeton, NJ, p.147–163.
Eisenberg, A., Seip, J.E., Gavagan, J.E., Payne, M.S., Anton, D.L., DiCosimo, R., 1997. Pyruvic acid production using methylotrophic yeast transformants as catalyst. J. Mol. Catal. B: Enzymatic, 2(4–5):223–232. [doi:10.1016/S1381-1177(96)00021-5]
Ghanem, N.B., Yusef, H.H., Mahrouse, H.K., 2000. Production of Aspergillus terreus xylanase in solid-state cultures: application of the Plackett-Burman experimental design to evaluate nutritional requirements. Bioresour. Technol., 73(2):113–121. [doi:10.1016/S0960-8524(99)00155-8]
Gokhade, D.V., Patil, S.G., Bastawde, K.B., 1991. Optimization of cellulase production by Aspergillus niger NCIM 1207. Appl. Biochem. Biotechnol., 30(2):99–109.
Hujanen, M., Linko, S., Linko, Y.Y., Leisola, M., 2001. Optimization of media and cultivation conditions for L. (+)(S)-lactic acid production by Lactobacillus casei NRRL B-441. Appl. Microbiol. Biotechnol., 56(1–2):126–130. [doi:10.1007/s002530000501]
Izumi, Y., Matsumura, Y., Tani, Y., Yamada, H., 1982. Pyruvic acid production from 1,2-propanediol by thiamin-requiring Acinetobacter sp. 80-M. Agric. Biol. Chem., 46(3):2673–2679.
Khuri, A.I., Cornell, J.A., 1987. Response Surfaces: Design and Analyses. Dekker, New York.
Lhomme, B., Roux, J.C., 1991. Utilization of experimental designs for optimization of Rhizopus arrhizus culture. Bioresour. Technol., 35(3):301–312. [doi:10.1016/09608524(91)90129-8]
Li, Y., Chen, J., Lun, S.Y., 2001a. Efficient pyruvate production by a multi-vitamin auxotroph of Torulopsis glabrata: key role and optimization of vitamin levels. Appl. Microbiol. Biotechnol., 55(6):680–685. [doi:10.1007/s002530100598]
Li, Y., Chen, J., Lun, S.Y., 2001b. Biotechnological production of pyruvic acid. Appl. Microbiol. Biotechnol., 57(4):451–459. [doi:10.1007/s002530100804]
Mahmoudian, M., Noble, D., Drake, C.S., Middleton, R.F., Montgomery, D.S., Picrcey, J.E., Ramlakhan, D., Todd, M., Dawson, M., 1997. An efficient process for production of N-acetylneuraminic acid using N-acetylneuraminic acid aldolase. Enzyme Microb. Technol., 20(5):393–400. [doi:10.1016/S0141-0229(96)00180-9]
Miyata, R., Yonehara, T., 1996. Improvement of fermentative production of pyruvate from glucose by Torulopsis glabrata IFO 0005. J. Ferment. Bioeng., 82(5):475–479. [doi:10.1016/S0922-338X(97)86986-3]
Ogawa, J., Soong, C.L., Masashi, I., Shimizu, S., 2001. Enzymatic production of pyruvate from fumarate—an application of microbial cyclic-imide-transforming pathway. J. Mol. Catal. B: Enzymatic, 11(4–6):355–359. [doi:10.1016/S1381-1177(00)00024-2]
Park, Y.S., Kang, S.W., Lee, J.S., Hong, S.I., Kim, S.W., 2002. Xylanase production in solid state fermertation by Aspergillus niger mutant using statistical experimental designs. Appl. Microbiol. Biotechnol., 58(6):761–766. [doi:10.1007/s00253-002-0965-0]
Plackett, R.L., Burman, J.P., 1946. The design of optimum multifactorial experiments. Biometrika, 33(4):305–325. [doi:10.2307/2332195]
Puri, S., Beg, Q.K., Gupta, R., 2002. Optimization of alkaline protease from Bacillus sp. by response surface methology. Curr. Microbiol., 44(4):286–290. [doi:10.1007/s00284-001-0006-8]
Rama Mohan Reddy, P., Reddy, G., Seenayya, G., 1999. Production of thermostable β-amylase and pullulanase by Clostridium thermosulfurogenes SV2 in solid-state fermentation: screening of nutrients using Plackett-Burman design. Bioprocess Eng., 21(2):175–179. [doi:10.1007/s004490050659]
Rosche, B., Leksawasdi, N., Sandford, V., Breuer, M., Hauer, B., Rogers, P., 2002. Enzymatic (R)-phenylacetylcarbinol production in benzaldehyde emulsions. Appl. Microbiol. Biotechnol., 60(1–2):94–100. [doi:10.1007/s00253-002-1084-7]
Roufs, J.B., 1996. Pyruvate: does it amp endurance and burn more fat? Muscle Fitness, 57(2):195–197.
Sadhukhan, A.K., Ramana Murthy, M.V., Ajaya Kumar, R., Mohan, E.V.S., Vandana, G., Bhar, C., Venkateswara Rao, K., 1999. Optimization of mycophenolic acid production in solid-state fermentation using response surface methodology. J. Ind. Microbiol. Biotechnol., 22(1):33–38. [doi:10.1038/sj.jim.2900597]
Schinschel, C., Simon, H., 1993. Preparation of pyruvate from (R)-lactate with Proteus species. J. Biotechnol., 31(2):191–203. [doi:10.1016/0168-1656(93)90160-O]
Sunitha, I., Subba Rao, M.V., Ayyanna, C., 1998. Optimization of medium constituents and fermentation conditions for the production of L-glutamic acid by the co-immobilized whole cells of Micrococcus glutamicus and Pseudomonas reptilivora. Bioprocess Eng., 18(5):353–359. [doi:10.1007/PL00008995]
Tsujino, T., Ohigashi, S., Sugiyama, S., Kawashiro, K., Hayashi, H., 1992. Oxidation of propylene glycol and lactic acid to pyruvic acid in aqueous phase catalyzed by lead modified alladium-on-carbon and related systems. J. Mol. Catal., 71(1):25–35. [doi:10.1016/0304-5102(92)80005-2]
Uchio, R., Kikuchi, K., Hirose, Y., 1976. Process for Producing Pyruvic Acid by Fermentation. US Patent, No. 3993 543.
Yokota, A., Schimizu, H., Terasawa, Y., Takaoka, N., 1994. Pyruvic acid production by a lipoic acid auxotroph of Escherichia coli W1485. Appl. Microbiol. Biotechnol., 41(6):638–643. [doi:10.1007/BF00167278]
Yonehara, T., Miyata, R., 1994. Fermentative production of pyruvate from glucose by Torulopsis glabrata. J. Ferment. Bioeng., 78(2):155–159. [doi:10.1016/0922-338X(94)90255-0]
Yu, X., Hallet, S.G., Sheppard, J., Watson, A.K., 1997. Application of the Plackett-Burman experimental design to evaluate nutritional requirements for the production of Collectotrichum coccodes spores. Appl. Microbiol. Biotechnol., 47(3):301–305. [doi:10.1007/s002530050930]
Zhang, J., Marcin, C., Shifflet, M.A., Salmon, P., Brix, T., Greasham, R., Buokland, B., Chartrain, M., 1996. Development of a defined medium fermentation process for physotigmine production by Streptomyces griseofuscus. Appl. Microbiol. Biotechnol., 44(5):568–575. [doi:10.1007/s002530050601]
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Zhang, J., Gao, Nf. Application of response surface methodology in medium optimization for pyruvic acid production of Torulopsis glabrata TP19 in batch fermentation. J. Zhejiang Univ. - Sci. B 8, 98–104 (2007). https://doi.org/10.1631/jzus.2007.B0098
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DOI: https://doi.org/10.1631/jzus.2007.B0098