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Identification of microbiota associated with Pectinatella magnifica in South Bohemia

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Abstract

The bacterial diversity of Pectinatella magnifica colonies sampled from pounds in South Bohemia during the summer of 2012 was investigated. The bacterial counts determined after cultivation on modified yeast extract-tryptone agar (Oxoid) supplemented with glucose (1 g L−1) varied from 4.22 to 6.61 and from 1.30 to 6.85 log CFU/g for aerobes and anaerobes, respectively. Higher counts were found in the superficial structures of Pectinatella colonies than in the inner gelled mass. Neither a trend in bacterial numbers at the individual site during the season, nor correlations between bacterial counts in P. magnifica and the surrounding water were observed. Fifty-four isolates were identified by sequencing the 16S rRNA gene and through MALDI-TOF MS analysis. Species of Aeromonas and Aquitalea were the predominantly isolated bacteria, but members of Chryseobacterium, Herbaspirillum, Enterobacter, Lactococcus, Leuconostoc, Pseudomonas and Sphingomonas were also found. As listed genera are wildly distributed in different water, soil, and plant samples, we conclude that Pectinatella colonies are inhabited by environmental bacteria. Nevertheless, a symbiotic relationship of these bacteria with P. magnifica cannot be excluded.

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Acknowledgements

This study was supported by a grant GACR P503/12/0337 of the Czech Science Foundation.

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Authors and Affiliations

  1. Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcka 129, CZ-16521, Prague 6 -Suchdol, Czech Republic

    Eva Vlková, Jiří Killer, Vojtěch Rada, Šárka Musilová, Věra Bunešová, Petra Hovorková, Matěj Božik & Hana Salmonová

  2. Institute of Animal Physiology and Genetics v.v.i., Academy of Sciences of the Czech Republic, Vídeňská 1083, CZ-14220, Prague 4 - Krč, Czech Republic

    Jiří Killer

  3. Institute of Animal Physiology of Slovak Academy of Sciences, Šoltesovej 4, SK-04001, Košice, Slovakia

    Vladimír Kmeť

  4. Department of Biology, Faculty of Agriculture, University of South Bohemia in České Budějovice, Studentská 13, CZ-37005, České Budějovice, Czech Republic

    Josef Rajchard

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  1. Eva Vlková
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Vlková, E., Killer, J., Kmeť, V. et al. Identification of microbiota associated with Pectinatella magnifica in South Bohemia. Biologia 70, 365–371 (2015). https://doi.org/10.1515/biolog-2015-0040

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